Applied Nmr Spectroscopy for Chemists and Life Scientists

From complex structure elucidation to biomolecular interactions - this applicationoriented textbook covers both theory and practice of modern NMR applications.
Part one sets the stage with a general description of NMR introducing important parameters such as the chemical shift and scalar or dipolar couplings. Part two describes the theory behind NMR, providing a profound understanding of the involved spin physics, deliberately kept shorter than in other NMR textbooks, and without a rigorous mathematical treatment of all the physico-chemical computations. Part three discusses technical and practical aspects of how to use NMR. Important phenomena such as relaxation, exchange, or the nuclear Overhauser effects and the methods of modern NMR spectroscopy including multidimensional experiments, solid state NMR, and the measurement of molecular interactions are the subject of part four. The final part explains the use of NMR for the structure determination of selected classes of complex biomolecules, from steroids to peptides or proteins, nucleic acids, and carbohydrates.
For chemists as well as users of NMR technology in the biological sciences.

Oliver Zerbe is the head of the NMR department at the University of Zurich. He studied chemistry and obtained his PhD under the supervision of Wolfgang von Philipsborn in Zurich. After a Postdoctoral stay in the group of Kurt W?thrich at the ETH Zurich he conducted his Habilitation at the Institute of Pharmaceutical Sciences at the ETH. In 2003 he returned to his present location at the University of Zurich. His main interests are in structural biology and in the structure of membrane proteins in particular. Oliver Zerbe is the author of approx. 80 scientific publications in peer-reviewed journals and has edited one book, 'NMR in drug research'.

INTRODUCTION
What type of information can be obtained from NMR spectra?
From 1D to 3D NMR

THEORY
Theory of the nuclear spin and the NMR experiment
The chemical shift
Scalar couplings constants
Nomenclature of Spin Systems
Proton and carbon NMR
Other nuclei
Relaxation (Dipolar Couplings) and Motion
The Nuclear Overhauser Effect (NOE)
Exchange Phenomena and Variable Temperature NMR
The Product Operator Formalism
2D NMR spectroscopy
3D NMR
Solid-State NMR Spectroscopy

PRACTICE
The components of an NMR spectrometer
Processing of spectra
Sample preparation
Choice of Solvents
Referencing
Determination of stereochemistry and optical purity
Hyphenation techniques (HPLC-NMR) and robotics
Shift tables for important chemical groups and solvents
Data bases

IDENTIFICATION AND STRUCTURE ELUCIDATION
Spectroscopic Identification of Natural Products and Synthetic Compounds
Typical Features in Spectra and Assignment Strategies for Terpenes, Steroids, Peptides, Carbohydrates, Alkaloids, Nucleic Acids

OTHER APPLICATIONS
Reaction control by NMR
Following enzymatic reactions by NMR
Rational drug design using NMR (SAR by NMR)
Screening techniques and molecular Interactions as studied by NMR