Bacteriophages

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

PrefaceContributorsIntroductionSection 1. Bacteriophage Isolation1. Methods for the Isolation of Viruses from Environmental SamplesK. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench, and Danielle M. Winget2. Bacteriophage Enrichment from Water and SoilRohan Van Twest and Andrew M. Kropinski3. Isolation of Phage via Induction of LysogensRaúl R. Raya and Elvira M. Hébert4. Isolation of Cyanophages from Aquatic EnvironmentsAndrew D. Millard 5. Isolation of Viruses from High Temperature EnvironmentsJennifer Fulton, Trevor Douglas, and Mark Young 6. Isolation of Novel Large and Aggregating BacteriophagesPhilip Serwer, Shirley J. Hayes, Julie Thomas, Borries Demeler, and Stephen C. Hardies Section 2. Bacteriophage Characterization 7. Enumeration of Bacteriophages by Double Agar Overlay Plaque AssayAndrew M. Kropinski, Amanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson8. Enumeration of Bacteriophages by the Direct Plating Plaque AssayAmanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson9. Enumeration of Bacteriophages Using the Small Drop Plaque Assay SystemAmanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson10. Determination of Virus Abundance by Epifluorescence MicroscopyAlice C. Ortmann and Curtis A. Suttle11. Enumeration of Bacteriophages Using Flow CytometryCorina P. D. Brussaard12. Basic Phage Electron MicroscopyHans-W. Ackermann13. Phage Classification and CharacterizationHans-W. Ackermann14. Phage Host Range and Efficiency of PlatingElizabeth Kutter 15. Measurement of the Rate of Attachment of Bacteriophage to Cells Andrew M. Kropinski16. Measurement of the Bacteriophage Inactivation Kinetics with Purified ReceptorsAndrew M. Kropinski17. Bacteriophage Plaques: Theory and AnalysisStephen T. Abedon and John Yin18. Practical Methods for Determining Phage Growth ParametersPaul Hyman and Stephen T. Abedon19. Phage Production and Maintenance of Stocks, Including Expected Stock LifetimesLouis-Charles Fortier and Sylvain Moineau Section 3. Bacteriophage-Host Interactions20. Construction of Phage Mutants (General)Robert Villafane21. Modifying Bacteriophage l with RecombineeringLynn C. Thomason, Amos B. Oppenheim, and Donald L. Court22. Identification and Isolation of Lysogens with Induced ProphageJonathan Livny, Christopher N. LaRock, and David I. Friedman23. Generalized TransductionAnne Thierauf, Gerardo Perez, and Stanley Maloy24. Preparation and Characterization of Polyclonal Anti-Phage SerumThomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, and Roger P. Johnson25. Generalized Transduction by Lytic BacteriophagesThomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Andrew M. Kropinski, and Roger P. Johnson