Biocatalysis for Practitioners Techniques, Reactions and Applications

This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Gonzalo de Gonzalo is a Ram�n y Cajal Researcher at the Departamento de Qu�mica at the University of Seville, Spain. He obtained his Degree and his Ph. D. in chemistry at the University of Oviedo (Spain). He spent his postdoctoral stage at Consiglio Nazionale delle Ricerche (Milan, Italy), moving back to University of Oviedo with a Juan de la Cierva Fellowship. In 2010, he spent one year at the University of Groningen working tin the research of novel oxidative enzymes. He worked two years for the pharmaceutical company Antibi�ticos S.A.U. (Le�n, Spain), moving to Seville in 2014. His research is focused on asymmetric synthesis by using different approaches, including biocatalytic and organocatalytic procedures, as well as the development of concurrent chemo- and biocatalytic reactions. He has published over 70 scientific publications and has recently been coeditor of the book "Biocatalysis: an Industrial Perspective".

Iv�n Lavandera completed his Ph. D. studies in Chemistry in 2003 with Prof. Vicente Gotor and Prof. Miguel Ferrero. He continued in Prof. Gotor's group as a researcher until 2005, and then he moved to the University of Graz as a postdoctoral researcher under the supervision of Prof. Wolfgang Kroutil. He returned to Oviedo in 2008, where he became first as a Clar�n and then as a Ram�n y Cajal post-doctoral researcher. Since 2015, he is Associate Professor at the Organic and Inorganic Department at the University of Oviedo, where he got the habilitation in 2017. He has been co-author of two patents and more than 90 publications. His research interests are focused on Biocatalysis, especially the use of oxidoreductases and transferases to achieve green processes and to develop new synthetic routes combining bio- and chemocatalysis in a concurrent manner.

Enzymatic commercial sources. Herein we will list the companies that offer biocatalysis nowadays. This section will be prepared by us.
Enzyme techniques
- Techniques for enzyme purifications
- Immobilization techniques for the preparation of supported biocatalysts
- Compartmentalization in biocatalysis
- Enzyme modification
Enzymes handling and applications
- Use of hydrolases in organic synthesis
- Enzymes applied to the synthesis of amines
- Oxidoreductases to obtain oxygenated compounds
- Synthase-catalyzed transformations
Ways to improve enzymatic transformations
- Application of non-conventional media in biocatalysis
- (Non-)conventional cofactor regeneration systems
- Flow biocatalysis
Recent trends in enzyme-catalyzed reactions
- Photobiocatalysis
- Multienzymatic systems
- Chemoenzymatic sequential protocols