Current Topics in Membranes: Peptide-Lipid Interactions

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9780121533526 | 0121533522
Cover: Hardcover
Copyright: 3/1/2002

eBook

Available Instantly

Online: 1825 Days

Downloadable: Lifetime Access

$241.88

This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion.

Contributors

xiii

Preface

xvii

Previous Volumes in Series

xix

PART I Biophysical Techniques for Analyzing Peptide-Lipid Interactions

Peptide-Membrane Interactions Determined Using Site-Directed Spin Labeling

David S. Cafiso

Introduction

(1)

Site-Directed Spin Labeling

(7)

Membrane Interactions of Basic Peptides Derived from Cell-Signaling Motifs

(6)

Studies Directed at Channel-Forming and Other Membrane-Active Peptides

(6)

Conclusions

(8)

References

(7)

Isothermal Titration Calorimetry for Studying Interactions between Peptides and Lipid Membranes

Torsten Wieprecht

Joachim Seelig

Introduction to Isothermal Titration Calorimetry

(1)

Measurement of the Reaction Enthalpy and the Binding Isotherm

(4)

Binding Models for Analyzing Binding Isotherms

(4)

Complex Membrane-Binding/Membrane-Perturbation Equilibria

(7)

Thermodynamics of the Membrane-Induced Coil-Helix Transition

(4)

Thermodynamics of Peptide Binding to Curved and Planar Membranes

(2)

Concluding Remarks

(4)

References

(4)

Infrared Reflection-Absorption Spectroscopy of Lipids, Peptides, and Proteins in Aqueous Monolayers

Richard Mendelsohn

Carol R. Flach

Introduction

(3)

Experimental Approaches

(5)

Information from IRRAS Measurements

(9)

IRRAS Applications to Lipid/Protein Langmuir Films

(8)

Current State and Future Prospects for IRRAS

(7)

References

(6)

Measuring the Depth of Amino Acid Residues in Membrane-Inserted Peptides by Fluorescence Quenching

Erwin London

Alexey S. Ladokhin

Introduction

(1)

Determining Depth from Fluorescence Quenching by Empirical Inspection of Quenching Level

(1)

Determining Depth by Parallax Analysis

(2)

Determining Depth by Distribution Analysis

(4)

Comparing Parallax Analysis and Distribution Analysis

(1)

Investigating Membrane Protein Topography by Varying Fluorophore Depth Instead of Quencher Depth

(1)

A Cautionary Note Concerning Quenching Analysis

(1)

Problems That Can Affect the Accuracy of Quenching Measurements

(1)

Studies of Membrane-Inserted Peptides by Fluorescence Quenching

100

(11)

Conclusions

111

(7)

References

111

(7)

Surface-Sensitive X-Ray and Neutron Scattering Characterization of Planar Lipid Model Membranes and Lipid/Peptide Interactions

Mathias Losche

Introduction

118

(4)

Data Inversion and Modeling in Reflectivity Measurements

122

(5)

Characterization of Planar Lipid Model Systems

127

(37)

References

150

(14)

Lipid-Peptide Interaction Investigated by NMR

Klaus Gawrisch

Bernd W. Koenig

Introduction

164

(1)

Peptide Structure in the Membrane-Bound State

165

(11)

Structure and Dynamics of the Lipid Matrix

176

(7)

Future Directions

183

(8)

References

184

(7)

Peptide-Lipid Interactions in Supported Monolayers and Bilayers

Lukas K. Tamm

Peptide Insertion into Lipid Monolayers

191

(3)

Supported Bilayers

194

(1)

ATR-FTIR Spectroscopy of Peptides in Supported Bilayers

195

(3)

Analysis of Fusion Peptide-Lipid Interactions

198

(8)

References

201

(5)

PART II Theoretical and Computational Analyses of Peptide-Lipid Interactions

Free Energy Determinants of Peptide Association with Lipid Bilayers

Amit Kessel

Nir Ben-Tal

Introduction

206

(5)

Theory

211

(12)

Mean-Field Studies of Peptide-Membrane Systems

223

(19)

Open Questions

242

(14)

References

244

(12)

Investigating Ion Channels using Computational Methods

Eric Jakobsson

R. Jay Mashl

Tsai-Tien Tseng

Introduction

256

(1)

Understanding Channel Function from Structure

257

(9)

Understanding Channel Function from Structural Homology

266

(5)

Future Prospects

271

(7)

References

271

(7)

PART III Experimental Investigations of Peptide-Lipid Interactions

The Role of Electrostatic and Nonpolar Interactions in the Association of Peripheral Proteins with Membranes

Diana Murray

Anna Arbuzova

Barry Honig

Stuart McLaughlin

Introduction

278

(1)

Electrostatic and Hydrophobic Interactions Mediate the Membrane Association of Important Biological Proteins

279

(3)

The Membrane Interaction of Simple Basic Peptides: Theory and Experiment

282

(7)

The Combination of Experimental and Theoretical Approaches Provides a Detailed Comprehensive Picture of the Membrane Association of Myristoylated Proteins

289

(11)

Future Directions

300

(10)

References

303

(7)

The Energetics of Peptide-Lipid Interactions: Modulation by Interfacial Dipoles and Cholesterol

Thomas J. McIntosh

Adriana Vidal

Sidney A. Simon

Introduction

310

(8)

Results

318

(9)

Discussion

327

(5)

Conclusions

332

(7)

References

333

(6)

Transmembrane α Helices

Sanjay Mall

J. Malcolm East

Anthony G. Lee

Intrinsic Membrane Proteins

339

(6)

Effects of Intrinsic Membrane Proteins on Lipid Bilayers

345

(6)

Model Transmembrane a Helices

351

(12)

Biological Consequences of Hydrophobic Mismatch

363

(9)

References

365

(7)

Lipidated Peptides as Tools for Understanding the Membrane Interactions of Lipid-Modified Proteins

John R. Silvius

Introduction

372

(1)

Structures of Lipid Modifications of Proteins

372

(3)

Chemistry of Peptide Lipidation

375

(2)

Thermodynamics of Association of Lipidated Peptides with Lipid Bilayers

377

(6)

Kinetics of Dissociation of Lipidated Sequences from Bilayers: Protein Targeting by Kinetic Trapping

383

(4)

Partitioning of Lipidated Peptides into Liquid-Ordered Lipid Domains

387

(2)

Conclusion

389

(9)

References

390

(8)

Experimental and Computational Studies of the Interactions of Amphipathic Peptides with Lipid Surfaces

Jere P. Segrest

Martin K. Jones

Vinod K. Mishra

G. M. Anantharamaiah

Introduction

398

(1)

Amphipathic α Helixes

398

(17)

Amphipathic β Strands/Sheets

415

(23)

References

430

(8)

Interactions of pH-Sensitive Peptides and Polymers with Lipid Bilayers: Binding and Membrane Stability

Doncho V. Zhelev

David Needham

Introduction

438

(2)

Interaction of Viral Fusion Peptides with the Membrane

440

(2)

Interaction of AcE4K with Lipid Bilayer Membranes (SOPC)

442

(8)

Effect of Peptide Binding on Membrane Stability

450

(6)

Membrane Instability Induced by the pH-Sensitive Polymer Poly(2-Ethylacrylic Acid) (PEAA)

456

(4)

Conclusion

460

(6)

References

460

(6)

The Hydrophobicity Threshold for Peptide Insertion into Membranes

Charles M. Deber

Li-Ping Liu

Chen Wang

Natalie K. Goto

Reinhart A. F. Reithmeier

Introduction

466

(1)

Results

467

(6)

Discussion

473

(3)

Conclusions

476

(8)

References

477

(7)

PART IV Specialized Topics of Biological Relevance

Signal Sequence Function in the Mammalian Endoplasmic Reticulum: A Biological Perspective

Christopher V. Nicchitta

Signal Sequences: Passage and Coronation

484

(1)

Signal Peptides: A Profile for Function

484

(3)

Signal Peptide Recognition: Where Biophysics Meets Biology

487

(5)

Yes, But What Happens at the Membrane?

492

(2)

Where to from Here?

494

(2)

Conclusion

496

(6)

References

496

(6)

The Process of Membrane Fusion: Nipples, Hemifusion, Pores, and Pore Growth

Fredric S. Cohen

Ruben M. Markosyan

Grigory B. Melikyan

Introduction

502

(1)

Structural Features and Conformational Changes of Fusion Proteins

503

(3)

Hemifusion and Its Possible Role in the Fusion Process

506

(2)

The Observed Temporal Sequence of Lipid Dye Spread and Fusion Pore Formation Depends upon Target Membrane and the Chosen Lipid Dye

508

(2)

The Dependence of Fusion on Spontaneous Curvature of Lipid Monolayers

510

(4)

An Ectodomain of HA Anchored to a Membrane Can Yield Fusion or End-State Hemifusion

514

(1)

Fusion Does Not Require a Precise Amino Acid Sequence of the TM Domain

515

(1)

Capturing Candidates of Transitional Hemifusion

515

(2)

The Role of Fusion Proteins in Hemifusion and Pore Formation

517

(1)

The Effects of Lipid Composition on Pore Flickering and Growth

518

(4)

Energy and Shape Considerations in Pore Growth

522

(2)

Outlook

524

(8)

References

524

(8)

Prenylation of CaaX-Type Proteins: Basic Principles through Clinical Applications

Herbert I. Hurwitz

Patrick J. Casey

Introduction

532

(2)

Enzymology of the CaaX Prenyltransferases: FTase and GGTase-I

534

(6)

Development of FTase Inhibitors as Anticancer Agents

540

(4)

Clinical Results from Evaluation of FTIs as Antitumor Agents

544

(1)

Summary and Conclusions

545

(7)

References

546

(6)

Peptides as Probes of Protein-Protein Interactions involved in Neurotransmitter Release

Thomas Kuner

Hiroshi Tokumaru

George J. Augustine

Introduction

552

(2)

Peptide Design

554

(8)

Characterization of Peptide Activity on Protein-Protein Interactions

562

(2)

Functional Analysis of Peptides in a Presynaptic Terminal

564

(3)

Summary and Outlook

567

(4)

References

568

(3)

Index

571

Please wait while the item is added to your bag...

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Current Topics in Membranes: Peptide-Lipid Interactions

Summary

Table of Contents

Supplemental Materials