Electroporation Protocols

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9781588298775 | 1588298779
Cover: Hardcover
Copyright: 1/30/2008

Rent

(Recommended)

$136.31

Term

Due

Price

Semester

Dec 13

$143.48

Quarter

Aug 16

$136.31

Short Term

Jul 4

$129.13

*This item is part of an exclusive publisher rental program and requires an additional convenience fee. This fee will be reflected in the shopping cart.
Buy Used

Usually Ships in 2-4 Business Days

$146.27
Buy New

Usually Ships in 7-10 Business Days

$196.03

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine , top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology'„¢ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.

Preface	p. v
Contributors	p. xiii
Basic Concepts of DNA Transfer via Electroporation
Application of Electroporation Gene Therapy: Past, Current, and Future	p. 3
Mechanism by Which Electroporation Mediates DNA Migration and Entry into Cells and Targeted Tissues	p. 19
Applicator and Electrode Design for In Vivo DNA Delivery by Electroporation	p. 35
Electrode Assemblies Used for Electroporation of Cultured Cells	p. 61
Formulations for DNA Delivery via Electroporation In Vivo	p. 77
Overview of Drug Delivery and Alternative Methods to Electroporation	p. 91
Nanoelectroporation: A First Look	p. 109
In Vitro Targeted Gene Delivery via Electroporation
Nucleofection of Human Embryonic Stem Cells	p. 131
Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination	p. 139
Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination	p. 155
Delivery of DNA into Natural Killer Cells for Immunotherapy	p. 165
Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication	p. 173
Delivery of DNA into Adipocytes within Adipose Tissue	p. 191
In Vivo Targeted Gene Delivery via Electroporation
Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges	p. 199
Delivery of DNA into Skeletal Muscle in Large Animals	p. 215
Delivery of DNA into Skin via Electroporation	p. 225
Electroporation-Mediated Gene Delivery to the Lungs	p. 233
Delivery of DNA into Bladder via Electroporation	p. 249
Analysis of Gene Function in the Retina	p. 259
Optical In Vivo Imaging of Electrically Mediated Delivery of siRNA into Muscle for Gene Function Analysis	p. 279
Electroporation of Adult Zebrafish	p. 289
Treatment of Cancer via Electroporation Gene Therapy
Flow Electroporation with Pulsed Electric Fields for Purging Tumor Cells	p. 301
Delivery of DNA into Tumors	p. 311
Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs	p. 319
Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation	p. 327
Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination	p. 339
Electroporation for Drug and Gene Delivery in the Clinic: Doctors Go Electric	p. 351
IL-2 Plasmid Electroporation: From Preclinical Studies to Phase I Clinical Trial	p. 361
Treatment of Other Diseases via Electroporation Gene Therapy
Factor IX Gene Therapy for Hemophilia	p. 375
KGF-1 for Wound Healing in Animal Models	p. 383
Hepatocyte Growth Factor Gene Therapy for Hypertension	p. 393
Electroporation of Corrective Nucleic Acids (CNA) In Vivo to Promote Gene Correction in Dystrophic Muscle	p. 405
Gene Delivery to Dystrophic Muscle	p. 421
Effect of Electroporation on Cardiac Electrophysiology	p. 433
Muscle and Fat Mass Modulation in Different Clinical Models	p. 449
Applications of Electroporation for DNA Vaccination
DNA Vaccination for Prostate Cancer	p. 463
HER2/neu DNA Vaccination for Breast Tumors	p. 473
Electroporation-Mediated HBV DNA Vaccination in Primate Models	p. 487
Taking Electroporation-Based Delivery of DNA Vaccination into Humans: A Generic Clinical Protocol	p. 497
Production of Monoclonal Antibody by DNA Immunization with Electroporation	p. 509
Index	p. 521
Table of Contents provided by Ingram. All Rights Reserved.

Please wait while the item is added to your bag...

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Electroporation Protocols

Summary

Table of Contents

Supplemental Materials