Guide to Techniques in Glycobiology

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis Among the topics covered: * Release of Oligosaccharides from Glycoproteins by Hydrazinolysis * Mass Spectrometry of Carbohydrate-Containing Biopolymers * High-resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides * Capillary Electrophoresis of Oligosaccharides * Synthesis and Uses of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs * Structural Analysis of Glycosylphosphatidylinositol Anchors * Identification of Polysialic Acids in Glycoconjugates