Introducing Proteomics From Concepts to

Introduction to Proteomics gives a concise and coherent overview of every aspect of current proteomics technology. The book provides students with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. Covering an Introduction, Separation Technologies, Analysis of Peptides/Proteins by Mass Spectrometry, and Strategies in Proteomics, this book also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, the book includes extensive references and a list of relevant proteomics information sources.

Josip Lovric is the author of Introducing Proteomics: From Concepts to Sample Separation, Mass Spectrometry and Data Analysis, published by Wiley.

Preface	p. ix
Acknowledgements	p. xi
Introduction	p. 1
What Are the Tasks in Proteomics?	p. 1
Challenges in Proteomics	p. 5
Proteomics in Relation to Other -omics and System Biology	p. 10
Some General Applications of Proteomics	p. 12
Structure of the Book	p. 18
References	p. 18
Separation and Detection Technologies	p. 21
Introduction to Experimental Strategies in Proteomics	p. 21
Gel-Based Separation	p. 31
Visualization and Analysis of Proteins/Peptides in Gels	p. 40
Gel-Free Separation Technologies	p. 54
Visualization of Proteins/Peptides from Hyphenated Methods	p. 74
Chips in Proteomic Applications	p. 81
References	p. 81
Analysis of Peptides/Proteins by Mass Spectrometry	p. 83
Basic Principles of Mass Spectrometry for Proteomics	p. 83
Ionization Methods for Small Amounts of Biomolecules	p. 101
Mass Analyzers and Mass Spectrometers	p. 116
Concluding Remarks on Mass Analyzers for Proteomics	p. 170
References	p. 170
Analysis and Interpretation of Mass Spectrometric and Proteomic Data	p. 173
Introduction	p. 173
Analysis of MS Data	p. 174
Analysis of MS/MS Data	p. 192
Quantification of LC MS and MS/MS Data from Complex Samples	p. 209
Bioinformatic Approaches for Mass Spectrometric Proteome Data Analysis	p. 213
References	p. 218
Strategies in Proteomics	p. 221
Imaging Mass Spectrometry	p. 221
Qualitative Proteomics	p. 223
Differential and Quantitative Proteomics	p. 234
Analysis of Posttranslational Modifications	p. 257
Interaction Proteomics	p. 261
Proteomics as Part of Integrated Approaches	p. 266
References	p. 271
Index	p. 275
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Introducing Proteomics From Concepts to Sample Separation, Mass Spectrometry and Data Analysis

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