Ion Channels: A Laboratory Manual

(preliminary)

Preface

Chapter 1
INTRODUCTION

Overview of Ion Channels

Chapter 2
INTRODUCTION

TRP Channels

Chapter 2
INTRODUCTION

Measuring Ca2+-Dependent Modulation of
Voltage-Gated Ca2+ Channels in HEK-293T cells
Jessica R. Thomas and Amy Lee

PROTOCOLS

Chapter 3
INTRODUCTION

Modulation of voltage-gated Ca2+ channels by G-proteins
Van B. Lu and Stephen R. Ikeda

PROTOCOL
G-Protein Modulation of Voltage-Gated Ca2+ Channels from Isolated
Adult Rat Superior Cervical Ganglion Neurons
Van B. Lu and Stephen R. Ikeda

Chapter 4
INTRODUCTION

Single Channel Recording of Ligand-Gated Ion Channels
Andrew J.R. Plested

PROTOCOL
Single-Channel Recording of Glycine Receptors in HEK Cells
Andrew J.R. Plested and Jelena Baranovic

Chapter 5
INTRODUCTION

Basic Physiological Properties of Synapses
Matthew A. Xu-Friedman

PROTOCOLS

Chapter 6
INTRODUCTION

Cholinergic Synaptic Signaling in the Thalamocortical System
M. Beirlein

Chapter 7
INTRODUCTION

In Vitro Investigation of Synaptic Plasticity
Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt, and
Per Jesper Sjöström

PROTOCOLS
Long-Term Potentiation by Theta-Burst Stimulation using Extracellular Field
Potential Recordings in Acute Hippocampal Slices
Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt, and
Per Jesper Sjöström

Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent
Plasticity in Acute Neocortical Slices
Txomin Lalanne, Therese Abrahamsson, and Per Jesper Sjöström

Chapter 8
INTRODUCTION

Voltage-gated Potassium Channels: A Structural Examination of Selectivity
and Gating
Dorothy M. Kim & Crina M. Nimigean

PROTOCOLS

Chapter 9
INTRODUCTION

Inhibition (Synapses/GABA/IPSCs)
Mark Farrant

PROTOCOLS

Chapter 10
INTRODUCTION

Whole-cell recording in the awake brain

PROTOCOLS

Chapter 11
INTRODUCTION

Stimulating neurons with light-gated channels

PROTOCOLS

Chapter 12
INTRODUCTION

HCN Currents in Neurons
Mala M. Shah

PROTOCOL
Protocol for Recording Ih in Neurons
Mala M. Shah

Chapter 13
INTRODUCTION

Combining Optogenetics and Electrophysiology to Analyze Projection
Neuron Circuits
Naoki Yamawaki, Benjamin A. Suter, Ian R. Wickersham, and
Gordon M. G. Shepherd

APPENDIX
General Safety and Hazardous Material Information

Index