Lab Experiments for General, Organic, and

This lab manual can accompany any text in general, organic and/or biochemistry. It is also available in a version that contains just the organic and biochemistry experiments.

Laboratory techniques: use of laboratory gas burner; basic glassworking

1

(10)

Laboratory measurements

11

(16)

Density determination

27

(12)

The separation of the components of a mixture

39

(10)

Resolution of a mixture by distillation

49

(8)

The empirical formula of a compound: the Law of Constant Composition:

57

(8)

Determination of the formula of a metal oxide

65

(8)

Classes of chemical reactions

73

(14)

Chemical properties of consumer products

87

(10)

Water analysis

97

(8)

Calorimetry: the determination of the specific heat of a metal

105

(16)

Boyle's Law: the pressure-volume relationship of a gas

121

(10)

Charles's Law: the volume-temperature relationship of a gas

131

(8)

Properties of gases: determination of the molecular weight of a volatile liquid

139

(10)

Physical properties of chemicals: melting point, sublimation, and boiling point

149

(12)

Entropy: a measure of disorder

161

(12)

Solubility and solution

173

(10)

Water of hydration

183

(10)

Colligative properties: freezing point depression and osmotic pressure

193

(10)

Factors affecting rate of reactions

203

(10)

The Law of Chemical Equilibrium and the Le Chatelier Principle

213

(8)

pH and buffer solutions

221

(10)

Analysis of vinegar by titration

231

(8)

Analysis of antacid tablets

239

(8)

Measurement of sulfur dioxide preservative in foods

247

(8)

Structure in organic compounds: use of molecular models. I

255

(14)

Stereochemistry: use of molecular models. II

269

(18)

Identification of hydrocarbons

287

(12)

Column and paper chromatography: separation of plant pigments

299

(12)

Identification of alcohols and phenols

311

(12)

Identification of aldehydes and ketones

323

(14)

Properties of carboxylic acids and esters

337

(12)

Properties of amines and amides

349

(10)

Polymerization reactions

359

(10)

Preparation of acetylsalicylic acid (aspirin)

369

(10)

Measurement of the active ingredient in aspirin pills

379

(6)

Isolation of caffeine from tea leaves

385

(10)

Carbohydrates

395

(10)

Preparation and properties of a soap

405

(8)

Preparation of a hand cream

413

(8)

Extraction and identification of fatty acids from corn oil

421

(8)

Analysis of lipids

429

(8)

TLC separation of amino acids

437

(10)

Acid-base properties of amino acids

447

(8)

Isolation and identification of casein

455

(12)

Isolation and identification of DNA from yeast

467

(8)

Viscosity and secondary structure of DNA

475

(12)

Kinetics of urease---catalyzed decomposition of urea

487

(10)

Isocitrate dehydrogenase---an enzyme of the citric acid cycle

497

(10)

Quantitative analysis of vitamin C contained in foods

507

(8)

Analysis of vitamin A in margarine

515

(10)

Urine analysis

525

(10)

Appendix 1 List of apparatus and equipment in student's locker

535

(1)

Appendix 2 List of common laboratory equipment and materials in the laboratory

536

(1)

Appendix 3 Special equipment and chemicals

537

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Lab Experiments for General, Organic, and Biochemistry

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Table of Contents

Supplemental Materials