Mass Spectrometry Instrumentation, Interpretation, and Applications

, by Ekman, Rolf; Silberring, Jerzy; Westman-Brinkmalm, Ann M.; Kraj, Agnieszka; Desiderio, Dominic M.; Nibbering, Nico M.

Write a Review

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9780471713951 | 0471713953
Cover: Hardcover
Copyright: 12/10/2008

Buy New

Print on Demand: 2-4 Weeks. This item cannot be cancelled or returned.

$175.42

With contributions from noted experts from Europe and North America, Mass Spectrometry Instrumentation, Interpretation, and Applications serves as a forum to introduce students to the whole world of mass spectrometry and to the many different perspectives that each scientific field brings to its use. The book emphasizes the use of this important analytical technique in many different fields, including applications for organic and inorganic chemistry, forensic science, biotechnology, and many other areas. After describing the history of mass spectrometry, the book moves on to discuss instrumentation, theory, and basic applications.

Foreword	p. xiii
Contributors	p. xv
Instrumentation	p. 1
Definitions and Explanations	p. 3
References	p. 13
A Mass Spectrometer's Building Blocks	p. 15
Ion Sources	p. 15
Gas Discharge	p. 16
Thermal Ionization	p. 16
Spark Source	p. 19
Glow Discharge	p. 20
Inductively Coupled Plasma	p. 21
Electron Ionization	p. 23
Chemical Ionization	p. 24
Atmospheric Pressure Chemical Ionization	p. 24
Photoionization	p. 25
Multiphoton Ionization	p. 25
Atmospheric Pressure Photoionization	p. 26
Field Ionization	p. 26
Field Desorption	p. 27
Thermospray Ionization	p. 27
Electrospray Ionization	p. 27
Desorption Electrospray Ionization	p. 29
Direct Analysis in Real Time	p. 30
Secondary Ion Mass Spectrometry	p. 31
Fast Atom Bombardment	p. 33
Plasma Desorption	p. 34
Laser Desorption/Ionization	p. 34
Matrix-Assisted Laser Desorption/Ionization	p. 35
Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization	p. 37
Mass Analyzers	p. 38
Time-of-Flight	p. 40
Magnetic/Electric Sector	p. 45
Quadrupole Mass Filter	p. 49
Quadrupole Ion Trap	p. 51
Orbitrap	p. 55
Fourier Transform Ion Cyclotron Resonance	p. 58
Accelerator Mass Spectrometry	p. 62
Detectors	p. 65
Photoplate Detector	p. 65
Faraday Detector	p. 67
Electron Multipliers	p. 67
Focal Plane Detector	p. 69
Scintillation Detector	p. 69
Cryogenic Detector	p. 70
Solid-State Detector	p. 70
Image Current Detection	p. 70
References	p. 71
Tandem Mass Spectrometry	p. 89
Tandem MS Analyzer Combinations	p. 91
Tandem-in-Space	p. 91
Tandem-in-Time	p. 95
Other Tandem MS Configurations	p. 97
Ion Activation Methods	p. 97
In-Source Decay	p. 97
Post-Source Decay	p. 98
Collision Induced/Activated Dissociation	p. 98
Photodissociation	p. 100
Blackbody Infrared Radiative Dissociation	p. 100
Electron Capture Dissociation	p. 101
Electron Transfer Dissociation	p. 101
Surface-Induced Dissociation	p. 101
References	p. 102
Separation Methods	p. 105
Chromatography	p. 106
Gas Chromatography	p. 106
Liquid Chromatography	p. 107
Supercritical Fluid Chromatography	p. 109
Electric-Field Driven Separations	p. 110
Ion Mobility	p. 110
Electrophoresis	p. 111
References	p. 113
Interpretation	p. 117
Introduction to Mass Spectra Interpretation: Organic Chemistry	p. 119
Basic Concepts	p. 119
Inlet Systems	p. 121
Direct Inlet	p. 121
Chromatography-Mass Spectrometry	p. 121
Physical Bases of Mass Spectrometry	p. 128
Electron Ionization	p. 129
Basics of Fragmentation Processes in Mass Spectrometry	p. 130
Metastable Ions	p. 135
Theoretical Rules and Approaches to Interpret Mass Spectra	p. 137
Stability of Charged and Neutral Particles	p. 137
The Concept of Charge and Unpaired Electron Localization	p. 148
Charge Remote Fragmentation	p. 151
Practical Approaches to Interpret Mass Spectra	p. 152
Molecular Ion	p. 152
High Resolution Mass Spectrometry	p. 155
Determination of the Elemental Composition of Ions on the Basis of Isotopic Peaks	p. 158
The Nitrogen Rule	p. 164
Establishing the [superscript 13]C Isotope Content in Natural Samples	p. 166
Calculation of the Isotopic Purity of Samples	p. 166
Fragment Ions	p. 168
Mass Spectral Libraries	p. 173
Additional Mass Spectral Information	p. 173
Fragmentation Scheme	p. 175
References	p. 177
Sequencing of Peptides and Proteins	p. 179
Basic Concepts	p. 179
Tandem Mass Spectrometry of Peptides and Proteins	p. 181
Peptide Fragmentation Nomenclature	p. 183
Roepstorff's Nomenclature	p. 183
Biemann's Nomenclature	p. 185
Cyclic Peptides	p. 187
Technical Aspects and Fragmentation Rules	p. 188
Why Peptide Sequencing?	p. 190
De Novo Sequencing	p. 192
Data Acquisition	p. 193
Sequencing Procedure Examples	p. 194
Tips and Tricks	p. 205
Peptide Derivatization Prior to Fragmentation	p. 207
Simplification of Fragmentation Patterns	p. 208
Stable Isotopes Labeling	p. 209
Acknowledgments	p. 210
References	p. 210
Online Tutorials	p. 210
Optimizing Sensitivity and Specificity in Mass Spectrometric Proteome Analysis	p. 211
Quantitation	p. 212
Peptide and Protein Identification	p. 213
Success Rate and Relative Dynamic Range	p. 218
Summary	p. 220
References	p. 220
Applications	p. 223
Doping Control	p. 225
References	p. 233
Oceanography	p. 235
References	p. 241
"OMICS" Applications	p. 243
Introduction	p. 243
Genomics and Transcriptomics	p. 246
Proteomics	p. 248
Metabolomics	p. 251
Space Sciences	p. 253
Introduction	p. 253
Origins	p. 254
Dynamics	p. 256
The Space MS Paradox	p. 257
A Brief History of Space MS	p. 259
Beginnings	p. 259
Linear TOF-MS	p. 260
Isochronous TOF-MS	p. 262
GENESIS and the Future	p. 264
References	p. 264
Bioterrorism	p. 267
What is Bioterrorism?	p. 267
Some Historical Accounts of Bioterrorism	p. 267
Geneva Protocol of 1925 and Biological Weapons Convention of 1972	p. 268
Categories of Biothreat Agents	p. 268
Challenges	p. 269
MS Identification of Biomarker Proteins	p. 270
Development of New Therapeutics and Vaccines Using Immunoproteomics	p. 271
References	p. 272
Imaging of Small Molecules	p. 275
SIMS Imaging	p. 277
Biological Applications (Cells, Tissues, and Pharmaceuticals)	p. 278
Catalysis	p. 280
Forensics	p. 281
Semiconductors	p. 282
The Future	p. 283
References	p. 285
Utilization of Mass Spectrometry in Clinical Chemistry	p. 287
Introduction	p. 287
Where are Mass Spectrometers Utilized in Clinical Applications?	p. 288
Most Common Analytes Detected by Mass Spectrometers	p. 288
Multianalyte Detection of Clinical Biomarkers, The Real Success Story	p. 289
Quantitative Profiling	p. 291
A Clinical Example of the Use of Mass Spectrometry	p. 292
Demonstrations of Concepts of Quantification in Clinical Chemistry	p. 294
Tandem Mass Spectrometry and Sorting (Pocket Change)	p. 294
Isotope Dilution and Quantification (the Jelly Bean Experiment)	p. 295
Polymers	p. 299
Introduction	p. 299
Instrumentation, Sample Preparation, and Matrices	p. 300
Analysis of Ultrapure Polymer Samples	p. 301
Analysis of Polymer Samples in which all Chains Possess the Same Backbone	p. 301
Analysis of Polymer Mixtures with Different Backbones	p. 303
Determination of Average Molar Masses	p. 303
References	p. 306
Forensic Sciences	p. 309
Introduction	p. 309
Materials Examined and Goals of Analysis	p. 311
Sample Preparation	p. 312
Systematic Toxicological Analysis	p. 312
GC-MS Procedures	p. 315
LC-MS Procedures	p. 315
Quantitative Analysis	p. 317
Identification of Arsons	p. 319
References	p. 319
New Approaches to Neurochemistry	p. 321
Introduction	p. 321
Why is there so Little Research in this Area?	p. 322
Proteomics and Neurochemistry	p. 323
The Synapse	p. 324
Learning and Memory	p. 324
The Brain and the Immune System	p. 325
Stress and Anxiety	p. 327
Psychiatric Diseases and Disorders	p. 329
Chronic Fatigue Syndrome	p. 329
Addiction	p. 330
Pain	p. 331
Neurodegenerative Diseases	p. 331
Conclusions	p. 333
Acknowledgments	p. 333
References	p. 334
Appendix	p. 337
Index	p. 353
Table of Contents provided by Ingram. All Rights Reserved.

Please wait while the item is added to your bag...

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Mass Spectrometry Instrumentation, Interpretation, and Applications

Summary

Author Biography

Table of Contents

Supplemental Materials