Methods in Cell Biology

Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. Describes proper specimen preparation for obtaining high-quality data and images Covers pitfalls in imaging different specimens and in employing different dyes Contains detailed protocols accessible to new users Chapters are self-contained units, but describe strategies that can be combined to develop new protocols Techniques are illustrated with full-color plates

S.J. Wright, V.E. Centonze, S.A. Stricker, P. Devries, S.W. Paddock, and G. Schatten, An Introduction to Confocal Microscopy and Three-Dimensional Reconstruction. J.J. Art and M.B. Goodman, Rapid Scanning Confocal Microscopy. L. Majlof and P.-O. Forsgren, Confocal Microscopy: Important Considerations for Accurate Imaging. T.C. Brelje, M.W. Weesendorf, and R.L. Sorenson, Multi-Color Laser Scanning Confocal Immunofluorescence Microscopy: Practical Application and Limitations. I. Kurtz and C. Emmons, Measurement of Intracellular pH with a Laser Scanning Confocal Microscope. L.M. Loew, Confocal Microscopy of Potentiometric Fluorescent Dyes. M. Terasaki and L. Jaffe, Imaging the EndoplasmicReticulum in Living Sea Urchin Eggs. A.H. Cornell-Bell, I.R. Otake, K. Sadler, P.G. Thomas, S. Lawrence, K. Olsen, F.R. Gumkowski, J.R. Peterson, and J.D. Jamieson, Membrane Glycolipid Trafficking in Living, Polarized Pancreatic Acinar Cells: Assessment by Confocal Microscopy. D.L. Gard, Confocal Immunofluorescence Microscopy of Microtubules in Amphibian Oocytes and Eggs. R.G. Summers, S.A. Stricker, and R.A. Cameron, Applications of Confocal Microscopy to Studies of Sea Urchin Embryogenesis. J.I. Hale and B. Matsumoto,Resolution of Subcellular Detail in Thick Tissue Sections: Immunohistochemical Preparation and Fluorescence Confocal Microscopy. G.E. Baker and B.E. Reese, Using Confocal Laser Scanning Microscopy to Investigate the Organization and Development of Neuronal Projections Labeled with DiI. J.N. Turner, J.W. Swann, D.H. Szaroski, K.L. Smith, D.O. Carpenter, and M. Fejtl,Three-Dimensional Confocal Light Microscopy of Neurons: Fluorescent and Reflection Stains.Chapter References. Subject Index.