Methods in Cell Biology Vol. 59 : The Zebrafish, Biology

, by Detrich, H. William; Matsudaira, Paul

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9780125441612 | 0125441614
Cover: Hardcover
Copyright: 1/1/1999

eBook

Available Instantly

Online: 1825 Days

Downloadable: Lifetime Access

$196.88

This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases

Contributors

xiii

(4)

Preface

xvii

PART I Introduction

(26)

1. Overview of the Zebrafish System

(8)

H. William Detrich, III

Monte Westerfield

Leonard I. Zon

I. Introduction

(1)

II. History of the Zebrafish System and Its Advantages and Disadvantages

(2)

III. Cell and Developmental Biology, Organogenesis, and Human Disease

(1)

IV. Genetics and Genomics

(1)

V. Future Prospects

(1)

VI. Conclusion

(1)

References

(3)

2. Cell Cycles and Development in the Embryonic Zebrafish

(18)

Donald A. Kane

I. Introduction

(1)

II. Terminology and the Staging Series

(1)

III. The Zygote Period

(2)

IV. The Cleavage Period

(2)

V. The Blastula Period

(8)

VI. The Gastrula Period

(1)

VII. The Segmentation Period

(1)

References

(4)

PART II Cell Culture and General Methods

(34)

3. Zebrafish Embryonal Cell Culture

(10)

Angela Helmrich

David Barnes

I. Introduction

(1)

II. General Cell Culture Considerations

(1)

III. Primary and Multipassage Zebrafish Embryo Cell Culture

(2)

IV. Transfection

(1)

V. Applications to Other Fish Models

(1)

VI. Conclusion

(1)

References

(3)

4. Primary Fibroblast Cell Culture

(7)

Barry H. Paw

Leonard I. Zon

I. Introduction

(1)

II. Material and Methods

(1)

III. Results and Discussion

(2)

References

(3)

5. Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)

(17)

Graham E. Corley-Smith

Bruce P. Brandhorst

Charline Walker

John H. Postlethwait

I. Introduction

(2)

II. Equipment and Materials

(2)

III. Methods

(4)

IV. Results and Discussion

(4)

V. Conclusions and Perspectives

(1)

References

(4)

PART III Gene Expression and Function in Development

(96)

6. Analysis of Protein and Gene Expression

(24)

Trevor Jowett

I. In Situ Hybridization to RNA and Immunolocalization of Proteins

(1)

II. Probe Synthesis

(3)

III. Fixation

(1)

IV. Hybridization to Whole-Mount Embryos

(3)

V. Immunolocalization of Probes

(2)

VI. Two-Color in Situ Hybridization

(4)

VII. Double-Fluorescent in Situ Hybridization

(1)

VIII. Simultaneous Localization of Transcription and Translation Gene Products

(4)

IX. Embedding and Sectioning Whole-Mount Embryos

(1)

X. Solutions and Reagents

(1)

References

(3)

7. Strategies to Perturb Zebrafish Development

(30)

Matthias Hammerschmidt

Patrick Blader

Uwe Strahle

I. Introduction

(1)

II. Administration of Reagents

(2)

III. Reagents

(5)

IV. Experiments to Modify the Activity of Specific Gene Products

(9)

V. Perspectives: Inducible Systems

104

(4)

References

108

(9)

8. Vectors and Techniques for Ectopic Gene Expression in Zebrafish

117

(11)

Tana M. Hyatt

Stephen C. Ekker

I. Introduction

117

(1)

II. Misexpression from DNA

118

(1)

III. Misexpression from RNA

118

(7)

References

125

(3)

9. Analysis of Zebrafish Development Using Explant Culture Assays

128

(31)

Yevgenya Grinblat

Mary Ellen Lane

Charles Sagerstrom

Hazel Sive

I. Introduction

128

(3)

II. Zebrafish Explants: General Considerations

131

(2)

III. Materials Required

133

(7)

IV. Guide to Explant Isolation and Culture

140

(10)

V. Using Explants to Assay Induction

150

(3)

VI. Illustrations of Specification and Induction Assays

153

(2)

VII. Future Directions

155

(1)

References

155

(4)

PART IV Early Embryonic Development

159

(94)

10. Embryonic Axis Formation in the Zebrafish

159

(21)

Mary C. Mullins

I. The First Signs of Dorsal

159

(1)

II. Establishment of Dorsal-Ventral Polarity in Blastula Stages

160

(1)

III. Dorsal-Ventral Patterning

161

(6)

IV. Development of Midline Mesodermal Tissues

167

(7)

References

174

(6)

11. Confocal Microscopic Analysis of Morphogenetic Movements

180

(26)

Mark S. Cooper

Leonard A. D'Amico

Clarissa A. Henry

I. Introduction

180

(1)

II. Confocal Imaging of Embryos

181

(1)

III. General Principles of Vital Staining

181

(11)

IV. Mounting Embryos for Imaging

192

(1)

V. Imaging Procedures

193

(2)

VI. Multilevel Time-Lapse Confocal Analysis

195

(5)

VII. Distribution of Visual Information

200

(1)

VIII. Confocal Imaging of Embryos Expressing Green Fluorescent Protein (GFP)

201

(1)

IX. Summary

201

(1)

References

202

(4)

12. Cytoskeletal Dynamics of the Zebrafish Embryo

206

(21)

Jacek Topezewski

Lilianna Solnica-Krezel

I. Introduction

206

(1)

II. Cytoskeleton of the Unfertilized Egg

206

(3)

III. Organization and Function of the Cytoskeleton in the Zygote

209

(3)

IV. Cleavage and Blastula Period

212

(3)

V. Yolk Cell Microtubules during Epiboly

215

(3)

VI. Tubulin Dynamics in Neuronal Axons of Living Zebrafish Embryos

218

(1)

VII. Methods

219

(5)

References

224

(3)

13. Kinesin-like Microtubule Motors in Early Development

227

(26)

Ming-Chyuan Chen

H. William Detrich, III

I. Introduction

227

(2)

II. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Cloning Strategy for Zebrafish Kinesin-like Protein Genes (zKLPs)

229

(6)

III. Production of Anti-zKLP Antibodies

235

(7)

IV. Expression of GFP-Tagged zKLPs in Cultured Cells

242

(5)

V. General Considerations

247

(1)

References

248

(5)

PART V Organogenesis

253

(134)

14. Techniques in Neural Development

253

(20)

Cecilia B. Moens

Andreas Fritz

I. Introduction

253

(1)

II. Organizers of Anterior-Posterior Pattern in the Neural Plate

254

(5)

III. Zebrafish Mutations That Disrupt A-P Pattern in the Neural Tube

259

(3)

IV. Techniques for the Study of Neural Patterning Mutants

262

(7)

Appendix: Codetection of Lineage Marker and Gene Expression in Whole-Mount Embryos

269

(1)

References

270

(3)

15. Development of the Retina

273

(29)

Jarema Malicki

I. Introduction

273

(2)

II. Development of the Zebrafish Retina

275

(3)

III. Embryological Analysis of Wild-Type and Mutant Zebrafish Visual Systems

278

(7)

IV. Genetic Analysis of the Zebrafish Retina

285

(9)

V. Summary

294

(1)

References

295

(7)

16. Growth Control in the Ontogenetic and Regenerating Zebrafish Fin

302

(12)

Stephen L. Johnson

Paul Bennett

I. Introduction

302

(2)

II. Mutations Affect the Growth, Development, and Regeneration of Fins

304

(3)

III. Developmental Checkpoints in Fin Growth

307

(1)

IV. Cellular Basis of Growth in the Fin

308

(2)

V. Conclusion

310

(1)

References

310

(4)

17. Vascular and Blood Gene Expression

314

(23)

Leon H. Parker

Leonard I. Zon

Didier Y.R. Stainier

I. Introduction

314

(1)

II. Embryonic Vascularization

314

(4)

III. Embryonic Hematopoiesis

318

(2)

IV. Vascular and Hematopoietic Gene Expression during Development

320

(7)

V. Mutations Affecting Vascularization and Hematopoiesis

327

(5)

VI. Conclusion and Future Directions

332

(1)

References

333

(4)

18. Analysis of Hemostasis in the Zebrafish

337

(22)

Pudur Jagadeeswaran

Yuan C. Liu

John P. Sheehan

I. Introduction

337

(6)

II. Methods

343

(9)

III. Future Assay Development and Applications

352

(3)

References

355

(4)

19. Cell Lineage Tracing in Heart Development

359

(8)

Fabrizio C. Serluca

Mark C. Fishman

I. Introduction

359

(1)

II. Methodology

360

(2)

III. Analysis

362

(2)

IV. Summary

364

(1)

References

364

(3)

20. Neurogenesis in Zebrafish Embryos

367

(20)

Ajay B. Chitnis

Igor B. Dawid

I. The System

367

(1)

II. Early Zebrafish Embryos Have a Simple Nervous System

368

(2)

III. Origins and Morphogenesis of the Nervous System

370

(1)

IV. Antagonists of Bone Morphogenetic Protein (BMP) Activity Lead to a Neural Fate in the Ectoderm

371

(1)

V. Fibroblast Growth Factor-8 (FGF-8) Defines Dorsoventral Positional Information in the Ectoderm

372

(1)

VI. Interactions with the Germ-Ring Provide Posteriorizing Signals

373

(1)

VII. Ablation of Cells in the Anterior Ectoderm Identifies a Novel Organizer

373

(1)

VIII. Patterning of the Neural Plate by Midline Signals: The Role of Factors in the Hedgehog, EGF, and TGF-Beta Families

373

(2)

IX. BMPs Induce Dorsal Neurons

375

(1)

X. Controlling the Number of Neurons: Proneuronal Domains and Proneural and Neurogenic Genes

375

(3)

XI. Position with Respect to Somite Boundaries Determines Motorneuron Subtype

378

(2)

XII. Patterning Neurogenesis in the Epiphysial Neurons

380

(1)

XIII. valentino (val) Suggests That Rhombomere Organization Is Preceded by the Formation of "Protosegments"

381

(1)

XIV. Perspectives

382

(1)

References

383

(4)

Index

387

Please wait while the item is added to your bag...

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Methods in Cell Biology Vol. 59 : The Zebrafish, Biology

Summary

Table of Contents

Supplemental Materials