Methods in Neurosciences Vol. 25 : Receptor

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Highlights * Cloning * Expression systems * Signal transduction * Structure-function techniques * Antireceptor antibodies * Regulation * 3-D receptor modeling and computational probing

Receptor Cloning
Approaches to Receptor Cloning
Purification of Receptors
Protein and Peptide Microsequencing: Applications in Neuroscience and Receptor Research
Xenopus Oocytes: A System for Expression Cloning and Structure--Function Studies of Ion Channels and Receptors
Cloning of G Protein-Coupled Opioid Receptors Using Degenerate PCR and Low Stringency Homology Screening
Hybrid Arrest Screening in Oocytes
Receptor Cloning: High-Throughput Sequencing of cDNA Tags for Identification of Novel Genes
Expression and Characterization
Ligand Binding and Second Messenger Assays for Cloned Gq/G11-Coupled Neuropeptide Receptors: The GnRH Receptor
Receptor Expression in Mammalian Cells
Baculovirus Expression of Receptors and Channels
Real-Time Measurements of Receptor Activity: Applications of Microphysiometric Techniques to Receptor Biology
Regulation of Na + -H + Exchange by G Protein-Coupled Receptors
Receptor-Activated Tyrosine Phosphatases: Activity Assays and Molecular Cloning
Studies of Functional Domains of Receptor and Channels
Site-Directed Mutagenesis
Receptor Chimers
Mapping G Protein Coupling Domains by Site-Specific Peptides
Synthesis and Expression of Synthetic Genes: Applications to Structure-Function Studies of Receptors
Use of Receptors Expressed in Escherichia coli to Study Autoimmunity against G Protein-Coupled Membrane Proteins
Integrated Methods for the Construction of Three Dimensional Models and Computational Probing of Structure-Function Relations in G-Protein Coupled Receptors
Localization and Regulation
Anti-fusion Protein Antibodies Specific for Receptor Subtypes
Development of Antireceptor Antibodies Using Synthetic Peptides
Receptor mRNA Measurement by Multiplex Nuclease Protection Assay
Antisense DNA/RNA-Based Strategies to Analysis of Signal Transduction via G Proteins
Index
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Methods in Neurosciences Vol. 25 : Receptor Molecular Biology

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Table of Contents

Supplemental Materials