Molecular Microbiology Diagnostic Principles and Practice

Presenting the latest molecular diagnostic techniques in one comprehensive volume

The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field.

Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.

Molecular Microbiology: Diagnostic Principles and Practice

• Presents the latest basic scientific theory underlying molecular diagnostics
• Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing
• Illustrates and summarizes key concepts and techniques with detailed figures and tables
• Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance
• Advises on the latest quality control and quality assurance measures
• Explores the increasing opportunities and capabilities of information technology

Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.

If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow.

Contributors

Preface

Section I: Novel and Emerging Technologies

1 Nucleic Acid Amplification Methods Overview

2 Application of Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory

3 Microbial Whole-Genome Sequencing: Applications in Clinical Microbiology and Public Health

4 Digital PCR and Its Potential Application to Microbiology

5 Massively Parallel DNA Sequencing and Microbiology

6 Next-Generation Sequencing

7 Pathogen Discovery

8 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Microbial Identification in Clinical Microbiology

9 Multiplex Technologies

Section II: Metagenomics: Implications for Diagnostics

10 The Skin Microbiome: Insights into Potential Impact on Diagnostic Practice

11 The Gastrointestinal Microbiome

12 The Vaginal Microbiome

13 Microbial Communities of the Male Urethra

14 The Human Virome in Health and Disease

Section III: Health Care-Associated Infections

15 Molecular Detection of Staphylococcus aureus Colonization and Infection

16 Molecular Diagnostics for Clostridium difficile

17 Overview of Molecular Diagnostics in Multiple-Drug-Resistant Organism Prevention: Focus on Multiple-Drug-Resistant Gram-Negative Bacterial Organisms

18 Detection of Vancomycin-Resistant Enterococci

Section IV: Molecular Diagnostics and Public Health

19 The Impact of Molecular Diagnostics on Surveillance of Foodborne Infections

20 Role of Molecular Methods in Improving Public Health Surveillance of Infections Caused by Antimicrobial-Resistant Bacteria in Health Care and Community Settings

21 Molecular Diagnostics: Huge Impact on the Improvement of Public Health in China

22 Surveillance and Epidemiology of Norovirus Infections

23 Molecular Diagnostic Assays for the Detection and Control of Zoonotic Diseases

Section V: Syndromic Diagnostics

24 Molecular Approaches to the Diagnosis of Meningitis and Encephalitis

25 Using Nucleic Acid Amplification Techniques in a Syndrome-Oriented Approach: Detection of Respiratory Agents

26 Molecular and Mass Spectrometry Detection and Identification of Causative Agents of Bloodstream Infections

27 Molecular Diagnosis of Gastrointestinal Infections

28 Diagnostic Approaches to Genitourinary Tract Infections

29 Syndromic Diagnostic Approaches to Bone and Joint Infections

Section VI: Virology

30 Molecular Detection and Characterization of Human Immunodeficiency Virus Type 1

31 Molecular Detection and Characterization of Hepatitis C Virus

32 Molecular Detection and Characterization of Hepatitis B Virus

33 Molecular Detection of Human Papillomaviruses

34 Molecular Diagnostics for Viral Infections in Transplant Recipients

Section VII: Fungi and Protozoa

35 Molecular Detection and Identification of Fungal Pathogens

36 Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi

37 Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance

38 Molecular Detection of Gastrointestinal Parasites

Section VIII: Point-of-Care/Near-Care Diagnostics

39 Molecular Diagnostics and the Changing Face of Point-of-Care

40 Point-of-Care Technologies for the Diagnosis of Active Tuberculosis

41 Molecular Diagnostics for Use in HIV/AIDS Care and Treatment in Resource-Limited Settings

42 Rapid Point-of-Care Diagnosis of Malaria and Dengue Infection

Section IX: The Host and Host Response

43 Implications of Pharmacogenetics for Antimicrobial Prescribing

44 Exploiting MicroRNA (miRNA) Profiles for Diagnostics

45 Host Response in Human Immunodeficiency Virus Infection

46 Biomarkers of Gastrointestinal Host Responses to Microbial Infections

Section X: Information Technology

47 Point-of-Care Medical Device Connectivity: Developing World Landscape

48 WHONET: Software for Surveillance of Infecting Microbes and Their Resistance to Antimicrobial Agents

49 Cloud-Based Surveillance, Connectivity, and Distribution of the GeneXpert Analyzers for Diagnosis of Tuberculosis (TB) and Multiple-Drug-Resistant TB in South Africa

Section XI: Quality Assurance

50 Molecular Method Verification

51 Molecular Microbiology Test Quality Assurance and Monitoring

52 Proficiency Testing and External Quality Assessment for Molecular Microbiology

53 Practices of Sequencing Quality Assurance

54 Verification and Validation of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry-Based Protocols

Section XII: The Business of Diagnostics

55 Improved Diagnostics in Microbiology: Developing a Business Case for Hospital Administration

56 Molecular Diagnostics and the Changing Legal Landscape

Index

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