Phage Display of Peptides and Proteins : A

Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references

Foreword
Biology of the Filamentous Bacteriophage
Principles and Applications of Phage Display
Vectros for Phage Display
Construction of Random Peptide Libraries in Bacteriophage
Construction and Screening of Antibody Display Libraries
Phagemeid-Displayed Peptide Libraries
Phage Libraries Displaying Random Peptides
Derived Form a Target Sequence
Display and Selection of Proteins on Genetic Packages
A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand
Interactions Made Possible By Linkage of Recogntion and Replication Functions
Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design
Use of Filamentous Virus Particles as Immunogens
Screening Phage Displayed Random Peptide Libraries
Phage Display Factors Affecting Planning Efficieny
Preparation of Second Generation Phage Libraries
Nonradioactive Sequencing of Random Peptide Recombinant Phage
Measurement of Peptide Binding Affinities Using Fluorescence Polarization
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Phage Display of Peptides and Proteins : A Laboratory Manual

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