A Practical Guide to the Study of Calcium in

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Key Features * Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes * fluorescent dyes * luminescent proteins * Includes techniques for perturbing intracellular Ca2+ * Covers detailed methodology plus problems and pitfalls of each technique * Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program * Color plates illustrate techniques such as * Confocal ratio-imaging * Use of aequorin

Ca²⁺ Buffers and [Ca²⁺]I Perturbation Techniques
A Practical Guide to the Preparation of Ca²⁺ Buffers
Photorelease Techniques for Raising or Lowering Intracellular Ca²⁺
Pharmacological Tools for Perturbing Intracellular Calcium Storage
Microelectrode Techniques for Measuring [Ca²⁺]I and Ca²⁺ Fluxes
How to Make and to Use Calcium-Specific Mini- and Microelectrodes
The Vibrating Ca²⁺
Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca²⁺ Influx and Efflux
IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels
Fluorescence Techniques for Imaging [Ca²⁺]I
Practical Aspects of Measuring [Ca²⁺] with Fluorescent Indicators
Rapid Simultaneous Estimation of Intracellular Calcium and pH
Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques
Confocal Imaging of Ca²⁺ in Cells
Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes
Use of Aequorin for [Ca²⁺]I Imaging
Inexpensive Techniques for Measuring [Ca²+]I Changes Using a Photomultiplier Tube
Imaging [Ca²⁺]I with Aequorin Using a Photon Imaging Detector
Targeting Recombinant Aequorin to Specific Intracellular Organelles
Index
Table of Contents provided by Publisher. All Rights Reserved.

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A Practical Guide to the Study of Calcium in Living Cells

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Table of Contents

Supplemental Materials