Practical High-Performance Liquid Chromatography

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9780470682180 | 0470682183
Cover: Hardcover
Copyright: 5/17/2010

Buy New

Print on Demand: 2-4 Weeks. This item cannot be cancelled or returned.

$214.23
eBook

Available Instantly

Online: 1825 Days

Downloadable: Lifetime Access

$74.25

This textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow manner. It contains a wealth of practical material and covers a wide range of subjects. Topics covered include principles, instrumentation, reverse-phase, ion-exchange, ion-pair, ion, size-exclusion and affinity chromatography, as well as analytical methods, preparative HPLC and enantiomeric separations. Appendices provide information on instrument testing, troubleshooting, column-packing.

Contents
Introduction
HPLC: a powerful separation method
A first HPLC experiment
Liquid chromatographic separation modes
The HPLC instrument
Safety in the HPLC laboratory
Comparison between high-performance liquid chromatography and gas chromatography
Comparison between high-performance liquid chromatography and capillary electrophoresis
Units for pressure, length, and viscosity
Scientific journals
Recommended books
Theoretical Principles
The chromatographic process
Band broadening
The chromatogram and its purport
Graphical representation of peak pairs with different degree of resolution¹¹
Factors affecting resolution
Extra-column volumes (dead volumes)¹⁵
Tailing
Peak capacity and statistical resolution probability¹⁹
Effects of temperature in hplc²³
The limits of hplc
How to obtain peak capacity
Pumps
General requirements
The short-stroke piston pump
Maintenance and repair
Other pump designs
Preparation of equipment up to sample injection
Selection of mobile phase
Preparation of the mobile phase
Gradient systems
Capillary tubing
Fittings¹²
Sample injectors
Sample solution and sample volume
Solvent properties
table of organic solvents
Solvent selectivity
Miscibility
Buffers³
Shelf-life of mobile phases
The mixing cross
Detectors
General
UV detectors²
Refractive index detectors
Fluorescence detectors
Electrochemical (amperometric) detectors³
Light scattering detectors⁶
Other detectors
Multiple detection
Indirect detection
Coupling with spectroscopy
Columns and stationary phases
Columns for HPLC
Precolumns
General properties of stationary phases¹
Silica⁴
Chemically modified silica⁶
Styrene-divinylbenzene¹³
Some other stationary phases
Column care and regeneration
HPLC column tests
Simple tests for HPLC columns
determination of particle size
Determination of breakthrough time
The test mixture
Dimensionless parameters for HPLC column characterization²
The van deemter equation from reduced parameters and its use in column diagnosis
diffusion coefficients³
Adsorption chromatography: normal-phase chromatography
What is adsorption?¹
The eluotropic series
Selectivity properties of the mobile phase
Choice and optimization of the mobile phase
Applications
Reversed-phase chromatography
Principle
Mobile phases in reversed-phase chromatography
Solvent selectivity and strength⁸
Stationary phases
method development in reversed-phase chromatography¹⁵
Applications
Hydrophobic interaction chromatography¹⁵
Chromatography with chemically bonded phases
introduction
properties of some stationary phases
hydrophilic interaction chromatography⁶
Ion-exchange chromatography
Introduction
Principle
Properties of ion exchangers
Influence of the mobile phase
Special possibilities of ion exchange
Practical hints
Applications
Ion-pair chromatography
Introduction
Ion-pair chromatography in practice
Applications
Appendix: UV detection using ion-pair reagents⁵
Ion chromatography¹
Principle
Suppression techniques
Phase systems³
Applications
Size-exclusion chromatography¹
Principle
The calibration chromatogram
Molecular mass determination by means of size-exclusion chromatography⁵
Coupled size-exclusion columns
Phase systems
Applications
Affinity chromatography
Principle¹
Affinity chromatography as a special case of hplc²
Applications
Choice of method
the various possibilities
method transfer⁸
Solving the elution problem
The elution problem
Solvent gradients²
Column switching⁷
Comprehensive two-dimensional hplc¹¹
Optimization of an isocratic chromatogram using four solvents
Optimization of the other parameters
Mixed stationary phases
Analytical HPLC
Qualitative analysis¹
Trace analysis
Quantitative analysis⁷
Recovery¹¹
Peak-height and peak-area determination for quantitative analysis¹⁵
Integration errors
The detection wavelength
Derivatization²⁰
Unexpected peaks: ghost and system peaks
Quality assurance¹
Is it worth the effort?
Verification with a second method
Method validation³
Standard operating procedures (SOP's)
Measurement uncertainty⁶
Qualifications, instrument test, and system suitability test
The quest for quality
Preparative hplc¹
Problem
Preparative hplc in practice²
Overloading effects
Fraction collection
Recycling
Displacement chromatography¹⁰
Separation of enantiomers¹
Introduction
Chiral mobile phases⁴
Chiral liquid stationary phases
Chiral solid stationary phases ⁶
Indirect separation of enantiomers¹⁶
Special possibilities
Micro, capillary and chip hplc¹
High-speed and super-speed hplc⁷
Fast separations at 1000 bar: uplc⁹
HPLC with supercritical mobile phases¹¹
HPLC with superheated water¹⁴
Electrochromatography¹⁵
Appendix 1: applied HPLC theory
Appendix 2: how to perform the instrument test
Introduction
Test sequence
Preparations
Pump test
UV detector test
Autosampler test
Column oven test
Equations and calculations
Documentation
Appendix 3: troubleshooting
Pressure problems
Leak in the pump system
Deviating retention times
Injection problems
Baseline problems
Peak shape problems
Problems with light scattering detectors (elsd)
Other causes
Instrument test
Appendix 4: column packing¹
Table of Contents provided by Publisher. All Rights Reserved.

Please wait while the item is added to your bag...

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Practical High-Performance Liquid Chromatography

Summary

Author Biography

Table of Contents

Supplemental Materials