Quantum Dots

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

Preface	p. v
Contributors	p. ix
Imaging of Fixed Cells and Tissues
Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugates	p. 3
Immunohistochemical Detection With Quantum Dots	p. 11
Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging	p. 29
Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dots	p. 43
Human Metaphase Chromosome FISH Using Quantum Dot Conjugates	p. 55
Imaging of Live Cells
Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell-Labeling of Membrane Receptors	p. 69
Single Quantum Dot Tracking of Membrane Receptors	p. 81
Optical Monitoring of Single Cells Using Quantum Dots	p. 93
Peptide-Mediated Intracellular Delivery of Quantum Dots	p. 105
Multiple Cell Lines Using Quantum Dots	p. 113
Measuring Cell Motility Using Quantum Dot Probes	p. 125
Imaging of Live Animals
Quantum Dots for In Vivo Molecular and Cellular Imaging	p. 135
Sentinel Lymph Node Mapping With Type-II Quantum Dots	p. 147
Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma	p. 161
Flow Cytometry Applications
Application of Quantum Dots to Multicolor Flow Cytometry	p. 175
Quantum Dots in Flow Cytometry	p. 185
Biochemical Applications
Luminescent Biocompatible Quantum Dots: A Tool for Immunosorbent Assay Design	p. 207
Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots	p. 229
Application of Quantum Dots to Multicolor Microarray Experiments: Four-Color Genotyping	p. 239
Index	p. 253
Table of Contents provided by Ingram. All Rights Reserved.

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