Salmonella

Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general.

Preface	p. v
Contributors	p. ix
Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex xMAP Suspension Array	p. 1
Detection of Salmonella by Bacteriophage Felix 01	p. 21
Physical Mapping of Salmonella Genomes	p. 39
Cloth-Based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella	p. 59
Genome-Based Identification and Molecular Analyses of Pathogenicity Islands and Genomic Islands in Salmonella enterica	p. 77
Determination of the Gene Content of Salmonella Genomes by Microarray Analysis	p. 89
In Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large Bacterial DNA Segments Using VEX-Capture	p. 105
Amplified Fragment Length Polymorphism Analysis of Salmonella enterica	p. 119
Salmonella Phages and Prophages: Genomics and Practical Aspects	p. 133
Salmonella Typhimurium Phage Typing for Pathogens	p. 177
Salmonella Phages Examined in the Electron Microscope	p. 213
Applications of Cell Imaging in Salmonella Research	p. 235
Analysis of Kinesin Accumulation on Salmonella-Containing Vacuoles	p. 275
Magnesium, Manganese, and Divalent Cation Transport Assays in Intact Cells	p. 289
Methods in Cell-to-Cell Signaling in Salmonella	p. 307
Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines	p. 323
Further Resources for Molecular Protocols in Salmonella Research	p. 355
Index	p. 359
Table of Contents provided by Ingram. All Rights Reserved.

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Summary

Table of Contents

Supplemental Materials