Methods in Cell Biology: Methods in Plant

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers.

Subcellular Fractionation and Analysis of Function
Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein
Functional Effects of Structural Changes in Photosystem II as Measured by Chlorophyll Fluorescence Kinetics
Determination of Protein Isoprenylation in Vitro and in Vivo
Extraction and Assay of Protein from Single Plant Cells
Photoaffinity Labeling and Strategies for Plasma Membrane Protein Purification
Cell Cycle Synchronization, Chromosome Isolation, and Flow-Sorting in Plants
Principles and Applications of Recombinant Antibody Phage Display Technology to Plant Biology
Isolation and Characterization of Plant Nuclei
Isolation of Nuclei Suitable for in Vitro
Transcriptional Studies
Analysis of H⁺-ATPase and Other Proteins of the Arabidopsis Plasma Membrane
Isolation and Reconstitution of the Vacuolar H⁺-ATPase
Isolation and Fractionation of Plant Mitochondria and Chloroplasts: Specific Examples
Isolating the Plant Mitotic Appratus: A Procedure for Isolating Spindles from the Diatom Cylindrotheca fusiformis
Chromoplasts
Methods for Isolation and Analysis of Polyribosomes
Methods for Isolation and Analysis of the Cytoskeleton
Isolation and Characterization of Plasmodesmata
Characterization and Isolation of the Chloroplast Protein Import Machinery
Macromolecular Movement into Mitochondria
Targeting of Proteins to the Nucleus
Import into the Endoplasmic Reticulum
Protein-Protein Interactions in the Endoplasmic Reticulum
Assaying Proteins for Molecular Chaperone Activity
The Use of Protoplasts to Study Protein Synthesis and Transport by the Plant Endomembrane System
Assessment of Translational Regulation by Run-Off Translation of Polysomesin Vitro
Molecular Methods for Analysis of Cell Function in Vivo
Electroporation of Plant Protoplasts and Tissues
Particle Bombardment
Preparation and Transformation of Monocot Protoplasts
Tobacco Protoplast Transformation and Use for Functional Analysis of Newly Isolated Genes and Gene Constructs
Novel Inducible/Repressible Gene Systems
Reporter Genes
Cell-Specific Ablation in Plants
Ribozymes
Expression of Plant Proteins in Baculoviral and Bacterial Systems
Expression and Localization of Plant Membrane Proteins in Saccharomyces
Synthesis of Plant Proteins in Heterologous Systems: Xenopus laevis Oocytes
Heterologous Expression of Higher Plant Transport Proteins and Repression of Endogenous Ion Currents in Xenopus Oocytes
Chapter References
Subject Index
Table of Contents provided by Publisher. All Rights Reserved.

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Methods in Cell Biology: Methods in Plant Cell Biology, Part A

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