Biotechnology Explorations

, by Scheppler, Judith A.; Cassin, Patricia E.; Gambier, Rosa M.

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ISBN: 9781555811785 | 1555811787
Cover: Paperback
Copyright: 4/1/2000

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A new college-level laboratory textbook of biotechnology experiments designed to provide students with hands-on understanding. The broad range of biotechnology experiments, including work with proteins, will provide students with valuable skills and an understanding of the applications of biotechnological theory. An ideal companion volume to a textbnook such as Glick and Pasternak's Molecular Biotechnology: Principles and Applications of Recombinant DNA.

Preface

vii

Acknowledgments

Laboratory Safety

(8)

The Fundamentals

(2)

SECTION I Getting Started

(38)

Documentation of Laboratory Experiments

(4)

Use of Equipment

(8)

Preparation of Solutions

(8)

Preparation and Maintenance of Microbial Cultures

(10)

Isolation of DNA by Spooling

(4)

Investigating the Structure and Bond Strength of DNA

(4)

SECTION II The Techniques

(48)

Restriction Enzyme Analysis of Lambda DNA

(10)

Miniprep of Plasmid DNA

(8)

Bacterial Transformation

(8)

Amplification of Human DNA by the Polymerase Chain Reaction

(10)

Quantitation of DNA by Spectrophotometry

(6)

Quantitation of DNA by Gel Electrophoresis

(6)

Applying the Fundamentals

(2)

SECTION III DNA Cloning

(42)

DNA Ligation and Cloning

(14)

DNA Cloning by Polymerase Chain Reaction

113

(14)

DNA Cloning for Protein Expression

127

(14)

SECTION IV Southern Blotting

141

(16)

Restriction Fragment Length Polymorphism Analysis of Human DNA

141

(16)

SECTION V Protein Studies

157

(30)

Quantitation of Protein

157

(6)

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Coomassie Blue Staining of Protein

163

(8)

Western Blotting and Immunodetection

171

(16)

SECTION VI Gene Transfer

187

(16)

Transformation of Yeasts

187

(8)

Transformation of Plants

195

(8)

SECTION VII Techniques for Studying Evolution and Systematics

203

(22)

Isolation of Genomic DNA

203

(12)

Comparison of DNAs from the Five Kingdoms

215

(4)

Evolution and Diversity through Protein Homologies

219

(6)

SECTION VIII Internet- and Computer-Assisted Gene Analysis

225

(26)

Internet Gene Bank Search

225

(8)

Computer-Assisted Sequence Analysis

233

(8)

Oligonucleotide Design by Computer

241

(10)

SECTION IX Advanced Techniques

251

(20)

DNA Sequencing

251

(8)

DNA Library Construction

259

(12)

Appendix

271

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Biotechnology Explorations

Summary

Table of Contents

Supplemental Materials