Handbook of Phycological Methods: Developmental and Cytological Methods

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ISBN: 9780521056632 | 0521056632
Cover: Paperback
Copyright: 3/24/2008

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The contributors include some of the most eminent specialists in the field of phycology. This volume focuses on cytological and developmental methods, each method already has been applied successfully to algae, and practical examples are given.

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Editor's preface
Introduction
Experimental Algal Systems and Techniques
Control of morphogenesis in Micrasterias
Control of development in Scenedesmus
Mating induction in Oedogonium
Gamete induction and flagellar adhesion in Chlamydomonas reinhardi
Acetabularia: techniques for study of nucleo-cytoplasmic interrelationships
Gamete release, fertilization, and embryogenesis in the Fucales
Hybridization and genetics of brown algae
Development in red algae: elongation and cell fusion
Production and selection of mutants in blue-green algae
Euglena: mutations, chloroplast 'bleaching', and differentiation
Crpthecodinium: sexual reproduction and mutagenesis
Protoplast and spheroplast production
Cytoplasts from coenocyic algal cells
Cytoplasmic streaming and membrane phenomena in cells of Characeae
Microbeam irradiation in Mougeotia
Phototropism: determination of an action spectrum in a tip-growing cell
Chromatic adaption in Fremyella diplosiphon and morphogenetic changes
How to detect the presence of a circadian rhythm
Light and Electron Microscopy: Preparative Methods
Photomicrography and special microscopic techniques
Polarized light, interference, and differential interference (Nomarski) optics
Preparation of algae for light microscopy Margaret
Fixation, embedding, sectioning, and staining of algae for electron microscopy Bernhard
Cytochemical localization
Quantitative cytochemical measurement of DNA in Eudorina (Chlorophyceae)
Autoradiography for light and electron microscopy
Immunochemistry: labeled antibodies
Freeze-fracture and freeze-etch techniques
Preparation of algae for scanning electron microscopy
Replica production and negative staining
Preparation of shadow-cast whole mounts
Stereology: quantitative electron microscopic analysis
Appendixes
Culture collections
List of suppliers
Indexes
Subject
Author
Taxonomic
Table of Contents provided by Publisher. All Rights Reserved.

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Handbook of Phycological Methods: Developmental and Cytological Methods

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