Finfish and Shellfish Bacteriology Manual Techniques and Procedures
, by Whitman, Kimberly A.Note: Supplemental materials are not guaranteed with Rental or Used book purchases.
- ISBN: 9780813819525 | 0813819520
- Cover: Spiral Bound
- Copyright: 10/20/2003
Significant advances in the field of microflora of aquatic animals, including fish and invertebrates, demands a practical laboratory text with the current methods for the bacteriological analysis of health and disease in fish and shellfish. Practical rather than theoretical, this manual is useful for individuals working in aquatic, environmental, and veterinary science, and in aquaculture, public health, and aquatic research. Methods are presented for the isolation and identification of bacterial pathogens in fish and shellfish and zoonotic pathogens in shellfish. The step-by-step presentation of procedures guides students in aquatic health and newcomers to the diagnostic laboratory, while providing a refresher course for the more experienced laboratory employee. Numerous tables and illustrations further assist readers. Thorough coverage of basic bacteriological techniques ensures that even the inexperienced aquatic health diagnostician will need no additional resources. The effective, easy-to-follow organization of the Finfish and Shellfish Bacteriology Manual make it the primary sourcebook for any laboratory. Whether it's used as an occasional quick reference by the experienced fish diagnostician, or as a tool to build diagnostic capabilities from the ground up, this book rises to the challenge.
Kimberley A. Whitman BSc., specializes in microbiology, and has 20 years experience working as a fish health bacteriologist/biologist with government agencies, academic institutions, and industry. Currently, Ms. Whitman is working with Malaspina University – College, Nanaimo, British Columbia as a fish health instructor. She also is employed with the private sector implementing a quality assurance program for a marine extraction plant, and is the owner of OHB Enterprises as a consultant for international aquatic facilities.
Preface, | xiii | ||||
Introduction, | xv | ||||
1. Finfish Bacteriology, | 3 | (4) | |||
2. Sample Collection to Detect Bacterial Infection in Finfish, | 7 | (4) | |||
3. Packaging Fish for Transport, | 11 | (4) | |||
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11 | (1) | |||
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12 | (3) | |||
4. Aseptic Bacterial Examination of Finfish, | 15 | (14) | |||
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15 | (1) | |||
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16 | (1) | |||
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17 | (1) | |||
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17 | (16) | |||
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17 | (1) | |||
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18 | (3) | |||
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18 | (1) | |||
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21 | (1) | |||
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22 | (4) | |||
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23 | (2) | |||
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25 | (1) | |||
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26 | (1) | |||
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27 | (1) | |||
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28 | (1) | |||
5. Shellfish Bacteriology, | 29 | (2) | |||
6. Sample Collection to Detect Bacterial Infection in Shellfish, | 31 | (2) | |||
7. Sampling and Packaging Bivalves and Crustaceans for Transport, | 33 | (4) | |||
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33 | (1) | |||
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34 | (3) | |||
8. Aseptic Bacterial Examination of Bivalves, | 37 | (10) | |||
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37 | (1) | |||
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37 | (1) | |||
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38 | (1) | |||
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38 | (1) | |||
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38 | (1) | |||
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39 | (2) | |||
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41 | (1) | |||
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42 | (2) | |||
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44 | (3) | |||
9. Aseptic Bacterial Examination of Lobsters, | 47 | (6) | |||
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47 | (1) | |||
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48 | (1) | |||
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48 | (1) | |||
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48 | (1) | |||
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49 | (1) | |||
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50 | (3) | |||
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50 | (1) | |||
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51 | (2) | |||
10. Diagnostic Methodology in Finfish and Shellfish Bacteriology, | 53 | (16) | |||
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53 | (1) | |||
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54 | (1) | |||
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59 | (2) | |||
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64 | (1) | |||
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65 | (4) | |||
11. Interpretation of Diagnostic Tests, | 69 | (12) | |||
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70 | (1) | |||
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71 | (1) | |||
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80 | (1) | |||
12. Bacteriology Culture Media, | 81 | (26) | |||
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81 | (23) | |||
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81 | (2) | |||
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83 | (1) | |||
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83 | (1) | |||
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84 | (1) | |||
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98 | (6) | |||
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104 | (1) | |||
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105 | (1) | |||
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105 | (2) | |||
13. Pure-Culture Techniques, | 107 | (12) | |||
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107 | (2) | |||
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109 | (10) | |||
14. Staining Methods, | 119 | (10) | |||
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120 | (1) | |||
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121 | (4) | |||
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125 | (4) | |||
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125 | (1) | |||
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126 | (3) | |||
15. Colony Identification, | 129 | (6) | |||
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129 | (2) | |||
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129 | (1) | |||
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130 | (1) | |||
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131 | (1) | |||
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132 | (1) | |||
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132 | (1) | |||
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133 | (2) | |||
16. Biochemical Activities of Bacteria, | 135 | (22) | |||
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135 | (3) | |||
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136 | (2) | |||
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138 | (1) | |||
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139 | (1) | |||
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140 | (2) | |||
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142 | (2) | |||
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152 | (5) | |||
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17. Serology, | 157 | ||||
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18. Antibiotic Testing, | 61 | (118) | |||
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162 | (15) | |||
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163 | (1) | |||
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163 | (5) | |||
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163 | (4) | |||
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167 | (1) | |||
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168 | (11) | |||
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168 | (11) | |||
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171 | (5) | |||
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176 | (1) | |||
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177 | (2) | |||
19. Specific Techniques Used for Finish Bacteriology, | 179 | (8) | |||
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179 | (3) | |||
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179 | (3) | |||
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180 | (2) | |||
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182 | (1) | |||
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182 | (2) | |||
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182 | (1) | |||
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185 | (1) | |||
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185 | (1) | |||
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185 | (1) | |||
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186 | (1) | |||
20. Specific Techniques Used for Shellfish Bacteriology, | 187 | (6) | |||
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187 | (2) | |||
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187 | (1) | |||
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187 | (1) | |||
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187 | (1) | |||
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188 | (1) | |||
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190 | (2) | |||
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192 | (1) | |||
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192 | (1) | |||
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192 | (1) | |||
21. Enumeration of Bacteria by the Plate Count Technique, | 193 | (4) | |||
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193 | (1) | |||
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194 | (1) | |||
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194 | (3) | |||
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195 | (1) | |||
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195 | (2) | |||
22. Public Health Indicator Organisms in Bivalves, | 197 | (20) | |||
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200 | (3) | |||
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200 | (1) | |||
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200 | (1) | |||
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201 | (1) | |||
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203 | (3) | |||
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206 | (6) | |||
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206 | (3) | |||
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206 | (1) | |||
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207 | (2) | |||
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207 | (2) | |||
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209 | (2) | |||
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209 | (1) | |||
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209 | (1) | |||
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210 | (3) | |||
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210 | (1) | |||
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211 | (1) | |||
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212 | (5) | |||
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213 | (1) | |||
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213 | (1) | |||
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214 | (3) | |||
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214 | (3) | |||
Appendix, | 217 | (1) | |||
Biochemistry Profiles of Disease-Causing Aquatic Organisms, | 217 | (1) | |||
Table A.1. Biochemistry profile Aerococcus viridans var. homari, | 218 | (1) | |||
Table A.2. Biochemistry profile Aeromonas hydrophila, | 219 | (1) | |||
Table A.3. Biochemistry profile Aeromonas salmonicida, subsp. salmonicida, | 220 | (1) | |||
Table A.4. Biochemistry profile atypical Aeromonas salmonicida, | 221 | (1) | |||
Table A.5. Biochemistry profile Aeromonas salmonicida, subsp. masoucida, | 222 | (1) | |||
Table A.6. Biochemistry profile Aeromonas salmonicida, subsp. achromogenes, | 223 | (1) | |||
Table A.7. Biochemistry profile Aeromonas salmonicida, subsp. smithia, | 224 | (1) | |||
Table A.8. Biochemistry profile Aeromonas sobria, | 225 | (1) | |||
Table A.9. Biochemistry profile Carnobacterium piscicola, | 226 | (1) | |||
Table A.10. Biochemistry profile Edwardsiella ictaluri, | 227 | (1) | |||
Table A.11. Biochemistry profile Edwardsiella tarda, | 228 | (1) | |||
Table A.12. Biochemistry profile Escherichia coli, | 229 | (1) | |||
Table A.13. Biochemistry profile Flavobacterium branchiophilum, | 230 | (1) | |||
Table A.14. Biochemistry profile Flavobacterium columnare, | 231 | (1) | |||
Table A.15. Biochemistry profile Flavobacterium hydatis, | 232 | (1) | |||
Table A.16. Biochemistry profile Flavobacterium psychrophilum, | 233 | (1) | |||
Table A.17. Biochemistry profile Hafnia alvei, | 234 | (1) | |||
Table A.18. Biochemistry profile Moritella viscosus, | 235 | (1) | |||
Table A.19. Biochemistry profile Photobacterium angustum, | 236 | (1) | |||
Table A.20. Biochemistry profile Photobacterium damselae subsp. damselae, | 237 | (1) | |||
Table A.21. Biochemistry profile Photobacterium damselae subsp. piscicida, | 238 | (1) | |||
Table A.22. Biochemistry profile Plesiomonas shigelloides, | 239 | (1) | |||
Table A.23. Biochemistry profile Pseudomonas aeruginosa, | 240 | (1) | |||
Table A.24. Biochemistry profile Pseudomonas fluorescens (Biovar I, II, III, IV, V), | 241 | (1) | |||
Table A.25. Biochemistry profile Renibacterium salmoninarum, | 242 | (1) | |||
Table A.26. Biochemistry profile Streptococcus iniae, | 243 | (1) | |||
Table A.27. Biochemistry profile Vibrio alginolyticus, | 244 | (1) | |||
Table A.28. Biochemistry profile Vibrio anguillarum (Serotype 01), | 245 | (1) | |||
Table A.29. Biochemistry profile Vibrio anguillarum (Serotype 02), | 246 | (1) | |||
Table A.30. Biochemistry profile Vibrio cholera, | 247 | (1) | |||
Table A.31. Biochemistry profile Vibrio fluvialis (Biovar I), | 248 | (1) | |||
Table A.32. Biochemistry profile Vibrio fluvialis (Biovar II), | 249 | (1) | |||
Table A.33. Biochemistry profile Vibrio ordalii, | 250 | (1) | |||
Table A.34. Biochemistry profile Vibrio parahemolyticus, | 251 | (1) | |||
Table A.35. Biochemistry profile Vibrio salmonicida, | 252 | (1) | |||
Table A.36. Biochemistry profile Vibrio vulnificus (Biotype II), | 253 | (1) | |||
Table A.37. Biochemistry profile Vibrio wodanis, | 254 | (1) | |||
Table A.38. Biochemistry profile Yersinia ruckeri (Type I), | 255 | (1) | |||
Table A.39. Biochemistry profile Yersinia ruckeri (Type II), | 256 | (1) | |||
Bibliography, | 257 |
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