Three-Dimensional Confocal Microscopy :

The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Key Features * Volume investigation * Three-dimensional reconstruction * Fluroescent probe design * Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology * Confocal data display and analysis * Twenty-eight pages of color

Preface
Confocal Microscopy
Practical Considerations: Introduction to Confocal Three-Dimensional Volume Investigation
Background Rejection and Optimization of Signal to Noise in Confocal Microscopy
Sources of Noise in Three-Dimensional Microscopical Data Sets
Simultaneous Ultraviolet and Visible Wavelength Confocal Microscopy
Fluorescent Labels for Confocal Microscopy
Display Methods for Gray-Scale, Voxel-Based Data Sets
Three-Dimensional Volume Reconstitution in Confocal Microscopy: Practical Considerations
Biological Applications of Confocal Microscopy: Imagine Ion Channels in Live Central Neurons Using Fluorescent Ligands
Imaging Ion Channels in Live Central Neurons Using Fluorescent Ligands II
Labeling of Cells and Tissues
Expression of G Protein-Coupled Receptors n Baculovirus/Sf9 Cells: Imaging Receptor Distribution by Confocal Fluorescence Microscopy
Dynamic Intracellular Calcium Compartments: Confocal Microscopy Using Fluo-3 in Cells and Organelles
Confocal Microscopy of Living Eggs and Embryos
Spatial Organization of Microtubules in PC12 Cells: Three-Dimensional Electron Microscopy and Confocal Microscopy Applied to Volume Investigation
Confocal Imaging of Living Neurons and Organelles
Confocal Microscopy in Diagnostic Cytology
Confocal Microscopy in Diagnostic Pathology
Alternative Approaches and Future Applications: Ultrathin Optical Sectioning and Dynamic Volume Investigation with Conventional Light Microscopy
Serial Electron Microscopy as an Alternative or Complement to Confocal Microscopy for the Study of Synapses and Dendritic Spines in the Central Nervous System
Confocal Scanning Opthalmoscopy: Applying Laser Scanning Technology to Retinal Imaging
Use of Three-Dimensional Reconstructed Magnetic Resonance Imaging Data for Neurosurgical Planning
Index
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Three-Dimensional Confocal Microscopy : Volume Investigation of Biological Specimens

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