Xenopus Laevis: Prepared Under the Auspices of the American Society for Cell Biology : Xenopus Laevis : Practical Uses in Cell and Molecula

This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all laboratories that use Xenopus. * Features approximately twenty full-color plates illustrating experimental techniques and results and depicting embryonic development * Provides complete coverage of Xenopus laevis as an experimental system including * Embryonic development, genetics, and laboratory care * Up-to-date protocols for experimental techniques using oocytes and embryos * General information listing recipes, suppliers, sequences, codons, and clones

General Information. M. Wu and J. Gerhart, Raising Xenopus in the Laboratory. J.D. Graf and H.R. Kobel, Genetics of Xenopus laevis. R. Tompkins and D. Reinschmidt, Experimentally Induced Homozygosity in Xenopus laevis. L.D. Smith, W. Xu, and R.L. Varnold, Oogenesis and Oocyte Isolation. R. Keller, Early Embryonic Development of Xenopus laevis. Oocytes. L.K. Opresko, Vitellogenin Uptake and in Vitro Culture of Oocytes. J.P. Evans and B.K. Kay, Biochemical Fractionation of Oocytes. J.G. Gall, H.G. Callan, Z. Wu, and C. Murphy, Lampbrush Chromosomes. M. Wormington, Preparation of Synthetic mRNAs and Analyses of Translational Efficiency of Micro-injected Xenopus Oocytes. C. Prives and D. Foukal, Use of Oligonucleotides for Antisense Experiments in Xenopus laevis Oocytes. Embryos. J. Heasman, S. Holwill, and C.C. Wylie, Fertilization of Cultured Xenopus Oocytes and Use in Studies of Maternally Inherited Molecules. J.L. Hedrick and D.M. Hardy, Isolation of Extracellular Matrix Structures from Xenopus laevis Oocytes, Eggs, and Embryos. D.G. Capco and W.M. Bement, Analysis of Cellular Signaling Events, the Cytoskeleton, and Spatial Organization of Macromolecules during Early Xenopus Development. K. Kao and M. Danilchik, Generation of Body Plan Phenotypes in Early Embryogenesis. R.L. Gimlich, Fluorescent Dextran Clonal Markers. J.B. Gurdon, Nuclear Transplantation in Xenopus. I.B. Dawid, Mesoderm Induction. C.R. Phillips, Neural Induction. T.D. Sargent and P.H. Mathers, Analysis of Class II Gene Regulation. P.D. Vize, A. Hemmati-Brivanlou, R.M. Harland, and D.A. Melton, Assays for Gene Function in Developing Xenopus Embryos. G.M. Kelly, D.W. Eib, and R.T. Moon, Histological Preparation of Xenopus laevis Oocytes and Embryos. M.W. Klymkowsky and J. Hanken, Whole-Mount Staining of Xenopus and Other Vertebrates. H.P. O'Keefe, D.A. Melton, B. Ferreiro, and C. Kintner, In Situ Hyridization. Model Systems Using Oocytes, Eggs, and Embryos. D. Carroll and C.W. Lehman, DNA Recombination and Repair in Oocytes, Eggs, and Extracts. A.L. Goldin, Expression of Ion Channels by Injection of mRNA into Xenopus Oocytes. H.B. Peng, L.P. Baker, and Q. Chen, Tissue Culture of Xenopus Neurons and Muscle Cells as a Model for Studying Synaptic Induction. D.W. DeSimone and K.E. Johnson, The Xenopus Embryo as a Model System for the Study of Cell-Extracellular Matrix Interactions. A.P. Wolffe and C. Schild, Chromatin Assembly. G.H. Leno and R.A. Laskey, DNA Replication in Cell-Free Extracts from Xenopus laevis. A.W. Murray, Cell Cycle Extracts. D.D. Newmeyer and K.L. Wilson, Egg Extracts for Nuclear Import and Nuclear Assembly Reactions. J.C. Smith and J.R. Tata, Xenopus Cell Lines. Appendixes H.B. Peng, Solutions and Protocols. B.K. Kay, Injections of Oocytes and Embryos. A. Droin, Mutants of Xenopus laevis. J.M. Cherry, Codon Usage for Xenopus laevis. M. Danilchik, H.B. Peng, and B.K. Kay, Pictorial Collage of Embryonic Stages. Xenopus Suppliers in the United States. R.M. Harland, In Situ Hyridization: An Improved Whole Mount Method for Xenopus Embryos. Chapter References. Index. Contents of Recent Volumes.