Advances in Chromatography: Volume 38

The 41st volume in the series Advances in Chromatography contains papers that take advantage of the properties of electroosmotic flow to explore capillary electrochromatography as an alternative to HPLC for nonvolatile analytes. The volume analyzes advances in biomembrane chromatography using reverse-phase HPLC for protein purification and biophysical studies of peptides and proteins at lipid-like surfaces and focuses on trace metal determination and speciation performed by liquid chromatography methods and more. The last chapter uses catechins in tea to illustrate concepts of chemical analysis.

A Tribute to J. Calvin Giddings

iii

Steven B. Giddings

Preface

ix

Contributors to Volume 38

xiii

Contents of Other Volumes

xix

Band Spreading in Chromatography: A Personal View.

1

(50)

John H. Knox

Column Structure

Early Theories

First Meeting with Calvin Giddings---Reduced Parameters

Obstructed Diffusion and the B-Term

Eddy Diffusion, Coupling, Radial Dispersion, and the Infinite Diameter Effect

Nonequilibrium Between the Stationary and Moving Zones or Phases

Separation of the A- and C-Terms

Exclusion Chromatography

Slow Chemical Equilibration and Wide Peaks

Band Spreading in Electroseparation Systems

Concluding Remarks

References

The Stochastic Theory of Chromatography

51

(24)

Francesco Dondi

Alberto Cavazzini

Maurizio Remelli

Introduction

Theoretical Approaches in Chromatography

Level One Theory: Structural Stochastic Concepts

Level Two Theory: Fundamentals and Basic Achievements of the Stochastic Theory of Chromatography

Level Three Theory: Some Applications of the Stochastic Theory to Experimental Peak Shape Analysis

The Characteristic Function Method in the Stochastic Theory of Chromatography

References

Solvating Gas Chromatography Using Packed Capillary Columns

75

(40)

Yufeng Shen

Milton L. Lee

Introduction

Packed-Column HPGC, SGC, SFC, and LC

Flow Transport in SGC

Efficiency in SGC

Separation Speed in SGC

Polarity and Solvating Power in SGC

References

The Linear-Solvent-Strength Model of Gradient Elution

115

(74)

L. R. Snyder

J. W. Dolan

Introduction

Liquid Chromatography Basics

The Linear-Solvent-Strength (LSS) Model

Optimizing Gradient Separation

Nonideal Effects in Gradient Elution

Application of the LSS Model to Gas Chromatography

Conclusion

References

Appendix: Isocratic Versus Gradient Resolution for Case of Unequal S-Values

High-Performance Liquid Chromatography-Pulsed Electrochemical Detection for the Analysis of Antibiotics

189

(44)

William R. LaCourse

Catherine O. Dasenbrock

Introduction

Aminoglycosides

Penicillins and Cephalosporins

Future Research

Conclusions

References

Theory of Capillary Zone Electrophoresis

233

(68)

H. Poppe

In Memory of J. Calvin Giddings

Introduction

Mobilities

Ions in Equilibrium

Electrophoresis in Capillaries

Electroosmotic Flow

Dispersion

Interaction of Ions

Effect of Electrolysis on Buffer Composition

Peak Integrals

References

Separation of DNA by Capillary Electrophoresis

301

(40)

Andras Guttman

Kathi J. Ulfelder

Introduction

Theory

Methods

Selected Applications

Future Directions

References

Index

341

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Advances in Chromatography: Volume 38

Summary

Table of Contents

Supplemental Materials