Heat Stress Tolerance in Plants Physiological, Molecular and Genetic Perspectives

Demystifies the genetic, biochemical, physiological, and molecular mechanisms underlying heat stress tolerance in plants

Heat stress—when high temperatures cause irreversible damage to plant function or development—severely impairs the growth and yield of agriculturally important crops. As the global population mounts and temperatures continue to rise, it is crucial to understand the biochemical, physiological, and molecular mechanisms of thermotolerance to develop ‘climate-smart’ crops. Heat Stress Tolerance in Plants provides a holistic, cross-disciplinary survey of the latest science in this important field. 

Presenting contributions from an international team of plant scientists and researchers, this text examines heat stress, its impact on crop plants, and various mechanisms to modulate tolerance levels. Topics include recent advances in molecular genetic approaches to increasing heat tolerance, the potential role of biochemical and molecular markers in screening germplasm for thermotolerance, and the use of next-generation sequencing to unravel the novel genes associated with defense and metabolite pathways. This insightful book:

• Places contemporary research on heat stress in plants within the context of global climate change and population growth
• Includes diverse analyses from physiological, biochemical, molecular, and genetic perspectives
• Explores various approaches to increasing heat tolerance in crops of high commercial value, such as cotton
• Discusses the applications of plant genomics in the development of thermotolerant ‘designer crops’ 

An important contribution to the field, Heat Stress Tolerance in Plants is an invaluable resource for scientists, academics, students, and researchers working in fields of pulse crop biochemistry, physiology, genetics, breeding, and biotechnology.

DR. SHABIR HUSSAIN WANI is Senior Assistant Professor, Department of Genetics and Plant Breeding, Mountain Research Centre for Field Crops, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.

DR. VINAY KUMAR is Assistant Professor, Department of Biotechnology, Modern College of Arts, Science and Commerce, Ganeshkhind, Pune, India, and a Visiting Faculty, Department of Environmental Sciences, Savitribai Phule University, Pune, India.

List of Contributors xiii

Foreword xix

About the Book xxi

About the Editor xxiii

1 Heat Tolerance in Cotton: Morphological, Physiological, and Genetic Perspectives 1
Muhammad Tehseen Azhar, Shabir Hussain Wani, Muhammad Tanees Chaudhary, Tariq Jameel, Parwinder Kaur, and Xiongming Du

1.1 Introduction 1

1.1.1 Morphological and Physiological Traits 2

1.1.1.1 Seedling and Root Growth 3

1.1.1.2 Stomatal Conductance 3

1.1.1.3 Cell Membrane Thermostability 4

1.1.1.4 Canopy Temperature 5

1.1.1.5 Chlorophyll Content 6

1.1.2 Genetics and Molecular Basis of Heat Tolerance in Cotton 8

1.1.3 Conventional Breeding Approaches 9

1.1.4 Modern Molecular Breeding Approaches 10

1.2 Conclusion and Future Prospects 12

References 12

2 Seed Priming as a Method to Generate Heat-stress Tolerance in Plants: A Minireview 23
Aditya Banerjee and Aryadeep Roychoudhury

2.1 Introduction 23

2.2 Mechanism of Heat Stress Injury in Plants 24

2.3 Seed Priming Generating Heat-stress Tolerance 26

2.4 Conclusion 27

2.5 Future Perspectives 27

Acknowledgments 28

References 28

3 How Effective are Stress-associated Proteins in Augmenting Thermotolerance? 33
Inès Karmous and Sandeep Kumar Verma

3.1 Introduction 33

3.1.1 Heat Shock Proteins (HSPs) 34

3.1.2 Proline 36

3.1.3 Dehydrins (DHNs) 37

3.1.4 Role of Metabolic Proteins in Thermotolerance 37

3.2 Conclusion 40

References 40

4 Biochemical and Molecular Markers: Unraveling Their Potential Role in Screening Germplasm for Thermotolerance 47
Ahmed Ismail, Kareem A. Mosa, Muna A. Ali, and Mohamed Helmy

4.1 Introduction 47

4.2 Types of Markers 48

4.3 Morphological Markers 49

4.4 Molecular Markers 49

4.5 Biochemical Markers 53

4.6 Quantitative Trait Loci for Plant Thermotolerance 54

4.7 Plant Metabolites Under Heat Stress 61

4.8 Antioxidant Enzymes and Heat Stress 63

4.9 Conclusion 68

References 70

5 Alteration in Carbohydrate Metabolism Modulates Thermotolerance of Plant under Heat Stress 77
Roseline Xalxo, Bhumika Yadu, Jipsi Chandra, Vibhuti Chandrakar, and S. Keshavkant

5.1 Introduction 77

5.1.1 Heat Stress and Thermotolerance 79

5.1.1.1 Morphological Alterations 80

5.1.1.2 Anatomical Alterations 80

5.1.1.3 Physiological and Biochemical Modifications 81

5.1.1.4 Cell Membrane Integrity 82

5.2 Carbohydrate as Protectives Molecules 83

5.2.1 Osmolyte 83

5.2.2 Thermoprotectant 84

5.3 Carbohydrates as Signaling Molecules 85

5.3.1 Reproductive Cell Development 85

5.3.2 Seed Development 86

5.3.3 Seed Germination and Yield Loss 87

5.4 Adverse Impacts of Heat Stress 87

5.4.1 Photosynthesis 87

5.4.1.1 Altered Carbon Assimilation 88

5.4.1.2 Chlorophyll Breakdown 88

5.4.2 Major and Minor Carbohydrate Metabolism 89

5.4.3 Expression of Regulatory Genes 89

5.4.4 Enzyme Activity 90

5.5 Mechanisms Involved in Thermotolerance 93

5.5.1 Glucose and Heat-stress Tolerance 93

5.5.2 Sucrose and Heat-stress Tolerance 94

5.5.3 Fructan and Heat-stress Tolerance 95

5.5.4 Trehalose and Heat-stress Tolerance 96

5.5.5 Raffinose and Heat-stress Tolerance 97

5.6 Genetic Approaches/Strategies for Improving Thermotolerance 97

5.6.1 Genetically Modified Crop Production 97

5.6.2 Transgenic Strategies 99

5.7 Conclusions and Future Perspectives 102

References 103

6 Transcriptomics to Dissect Plant Responses to Heat Stress 117
Sagar Satish Datir

6.1 Introduction 117

6.1.1 Transcriptome Sequencing and Expression Profiling of Genes Involved in Heat Stress Response 119

6.1.1.1 Rice (Oryza sativa L.) 119

6.1.1.2 Wheat (Triticum aestivum L.) 123

6.1.1.3 Maize (Zea mays L.) 125

6.1.1.4 Switchgrass (Panicum virgatum L.) and Ryegrass (Lolium perenne L.) 126

6.1.1.5 Spinach (Spinacia oleracea L.) 128

6.1.1.6 Brassica rapa L. 129

6.1.1.7 Banana (Musa acuminate Colla) 130

6.2 Conclusions 131

References 132

7 Proteomics as a Tool for Characterizing the Alteration in Pathways Associated with Defense and Metabolite Synthesis 141
Reetika Mahajan and Sajad Majeed Zargar

7.1 Introduction 141

7.2 What Is Proteomics? 142

7.3 Need of Proteomics in Post-genomic Era 143

7.4 Different Branches of Proteomics 143

7.5 Techniques Used in Quantitative Proteomics 145

7.5.1 Gel-based and Gel-free Methods 146

7.5.2 Label-based and Label-free Methods 149

7.6 Role of Proteomics in Studying Alteration in Pathways Associated with Defense and Metabolite Synthesis 150

7.7 Conclusion and Future Perspective 156

References 156

8 RNA World and Heat Stress Tolerance in Plants 167
Usman Ijaz, Muhammad Amjad Ali, Habibullah Nadeem, Lin Tan, and Farrukh Azeem

8.1 Introduction 167

8.2 Plant microRNAs 168

8.3 Small Interfering RNA (siRNA) 177

8.4 Long Noncoding RNAs (lncRNAs) 178

8.5 Circular RNAs (circRNAs) 179

8.6 Conclusions and Future Perspectives 180

References 180

9 Heat Shock Proteins: Master Players for Heat-stress Tolerance in Plants during Climate Change 189
Annu Yadav, Jitender Singh, Koushlesh Ranjan, Pankaj Kumar, Shivani Khanna, Madhuri Gupta, Vinay Kumar, Shabir Hussain Wani, and Anil Sirohi

9.1 Introduction 189

9.2 Classification of HSPs 192

9.2.1 HSP100 192

9.2.1.1 Structure of HSP100 192

9.2.1.2 Mode of Action: The HSP100 Chaperone Cycle 192

9.2.2 HSP90 195

9.2.2.1 Structure of HSP90 197

9.2.2.2 Mode of Action: The HSP90 Chaperone Cycle 197

9.2.3 HSP70 198

9.2.3.1 Structure of HSP70 198

9.2.3.2 Mode of Action: The Hsp70 Chaperone Cycle 198

9.2.4 HSP60 199

9.2.4.1 Structure of HSP60 201

9.2.4.2 Mode of Action: The Hsp60 Chaperone Cycle 201

9.2.5 The Small Heat Shock Protein Family (sHSPs) 202

9.2.5.1 Structure of sHSP 202

9.2.5.2 Mode of Action: Small Heat Shock Proteins 203

9.3 HSPs Expression Under Heat Stress Condition 203

9.4 Conclusion and Future Prospects 205

References 205

10 The Contribution of Phytohormones in Plant Thermotolerance 213
Sonal Mishra, Mansi Bhardwaj, Shakti Mehrotra, Aksar Ali Chowdhary, and Vikas Srivastava

10.1 Introduction 213

10.2 Protectants in Heat Stress Alleviation 215

10.2.1 Osmolytes 215

10.2.2 Nutrients 215

10.2.3 Signaling Molecules 217

10.2.4 Polyamines 217

10.2.5 Phytohormones 218

10.3 Application of Hormones in HT Management 218

10.3.1 Auxin 219

10.3.2 Gibberellin 221

10.3.3 Cytokinin 222

10.3.4 Abscisic Acid 224

10.3.5 Ethylene 225

10.3.6 Salicylic Acid 225

10.3.7 Jasmonic Acid 227

10.3.8 Brassinosteroid 228

10.4 Conclusion and Prospects 229

Acknowledgements 229

References 230

11 Exploring In-built Defense Mechanisms in Plants under Heat Stress 239
Giridara Kumar Surabhi and Jatindra Kumar Seth

11.1 Introduction 239

11.2 Effect of Heat Stress on Crop Plants 240

11.2.1 Heat Stress Effects on Physiology and Cell Structures 241

11.2.2 Heat Stress Effects on Vegetative Stages 242

11.2.3 Heat Stress Effects on Reproductive Stage 243

11.2.4 Heat Stress Effects on Yield 243

11.3 Threshold Temperature 244

11.4 In-built Defense System in Plants to Overcome High Temperature Stress 245

11.4.1 Accumulation of Thermoprotectants 245

11.4.1.1 Heat Shock Proteins (HSPs) 245

11.4.1.2 Proline 246

11.4.1.3 Glycinebetaine (GB) 248

11.4.1.4 Abscisic Acid (ABA) 249

11.4.1.5 Salicylic Acid (SA) 250

11.4.1.6 Heat Stress Effects on Secondary Metabolism 255

11.4.2 Transcriptional Regulation 256

11.4.3 Role of Small RNAs (miRNAs) in Heat-stress Tolerance 258

11.5 Conclusion 261

Acknowledgement 262

References 263

Index 283

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