High Performance Liquid Chromatography

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Introduction. Efficiency, Retention, Selectivity, and Resolution in Chromatography. Modes of Chromatography. Support Materials and Solvents. Instrumentation: Pumps, Injectors, and Column Design. Instrumentation: Detectors and Integrators. Method Development and Quantitation. Sample Preparation. Polymer Analysis. HPLC in Biomedical and Forensic Analysis. Environmental Analysis. Food, Organic, and Pharmaceutical Applications.