7. Chemically Defined, Complex, Selective, and Differential Media
8. Quantification of Microorganisms
Introduction to the Environment and Microbial Growth
9. Aerobic and Anaerobic Growth
10. The Effect of Incubation Temperature on Generation Time
Introduction to Control of Microbial Growth
11. Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time
12. Control of Microbial Growth with Ultraviolet Light
13. Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth
14. Antibiotics
15. Antiseptics and Disinfectants
Introduction to Microbial Genetics
16. Selection of Bacterial Mutants Resistant to Antibiotics
17. Transformation: A Form of Genetic Recombination
18. Gene Regulation: Induction and Catabolite Repression
PART TWO
The Other Microbial World
Introduction to the Other Microbial World
l9. Microscopic Identification of Fungi
20. Parasitology: Protozoa and Helminths
21. Titering Prokaryotic Viruses
PART THREE
Microbes and Humans
Introduction to Medical Microbiology
22. Normal Skin Biota
23. Streptococci and Respiratory Microorganisms
24. Identification of Enteric Gram-Negative Rods
25. Clinical Unknown Identification
PART FOUR
Immunology
Introduction to Some Immunological Principles and Techniques
26. Differential White Blood Cell Stains
27. Lysozyme, an Enzyme Contributing to Innate Immunity
28. Enzyme-Linked Immunoabsorbent Assay (ELISA)
PART FIVE
Public Health
Introduction to Public Health
29. Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques
30. Microbial Examination of Food
31. Epidemiology
PART SIX
Applications of Biotechnology
Introduction to Biotechnology
32. Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR)
33. Electrophoresis
34. Identification of Bacteria Using the Ribosomal Database Project
35. Microarray
PART SEVEN
Individual Projects
Introduction to the Individual Projects
36. Luminescent Bacteria: Bacteria That Produce Light
APPENDICES
1. Culture Media and Reagent Formulas
2. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual
3. Dilution Practice Problems
4. Alternative Procedures
5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size
6. History and Working Principles of Light Microscopy
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