- ISBN: 9781621821311 | 1621821315
- Cover: Paperback
- Copyright: 3/23/2016
(preliminary)
Preface
Chapter 1
INTRODUCTION
Overview of CRISPR/Cas9 Biology
Hannah K. Ratner, Timothy R. Sampson, and David S. Weiss
Chapter 2
INTRODUCTION
Guide RNAs: A Glimpse at the Sequences that Drive CRISPR-Cas Systems
Alexandra E. Briner and Rudolphe Barrangou
PROTOCOL
Prediction and Validation of Native and Engineered Cas9 Guide Sequences
Alexandra E. Briner, Emily D. Henriksen, and Rodolphe Barrangou
Chapter 3
INTRODUCTION
Characterization of Cas9-gRNA Orthologs
Jonathan L. Braff, Stephanie J. Yaung, Kevin M. Esvelt, and
George M. Church
PROTOCOL
Characterizing Cas9 PAMs with High-Throughput Sequencing of Library
Depletion Experiments
Jonathan L. Braff, Stephanie J. Yaung, Kevin M. Esvelt, and
George M. Church
Chapter 4
INTRODUCTION
Large-Scale Single-Guide RNA Library Construction and Use for
CRISPR-Cas9-Based Genetic Screens
Tim Wang, Eric S. Lander, and David M. Sabatini
PROTOCOLS
Single-Guide RNA Library Design and Construction
Tim Wang, Eric S. Lander, and David M. Sabatini
Viral Packaging and Cell Culture for CRISPR-Based Screens
Tim Wang, Eric S. Lander, and David M. Sabatini
Chapter 5
PROTOCOL
AAV-Mediated Delivery of CRISPR-Cas Systems for Genome Engineering
in Mammalian Cells
Thomas Gaj and David V. Schaffer
Chapter 6
INTRODUCTION
Detecting Single-Nucleotide Substitutions Induced by Genome Editing
Yuichiro Miyaoka, Amanda H. Chan, and Bruce R. Conklin
PROTOCOL
Using Digital PCR to Detect Single-Nucleotide Substitutions Induced
by Genome Editing
Yuichiro Miyaoka, Amanda H. Chan, and Bruce R. Conklin
Chapter 7
PROTOCOL
CRISPR-Cas9 Genome Engineering in Saccharomyces cerevisiae Cells
Owen W. Ryan, Snigdha Poddar, and Jamie H. D. Cate
Chapter 8
INTRODUCTION
Cas9-Mediated Genome Engineering in Drosophila melanogaster
Benjamin Housden, Yanhui Hu, and Norbert Perrimon
PROTOCOLS
Design and Generation of Donor Constructs
Benjamin E. Housden and Norbert Perrimon
Detection of Indel Mutations in Drosophila by High-Resolution
Melt Analysis (HRMA)
Benjamin E. Housden and Norbert Perrimon
Design and Generation of Drosophila sgRNA Expression Constructs
Benjamin E. Housden, Yanhui Hu, and Norbert Perrimon
Chapter 9
INTRODUCTION
Optimization Strategies for the CRISPR-Cas9 Genome Editing System
Charles E. Vejnar, Miguel Moreno-Mateos, Daniel Cifuentes,
Ariel A. Bazzini, and Antonio J. Giraldez
PROTOCOL
Optimized Strategies for the CRISPR-Cas9 System for Genome Editing in Zebrafish
Charles E. Vejnar, Miguel Moreno-Mateos, Daniel Cifuentes,
Ariel A. Bazzini, and Antonio J.Giraldez
Chapter 10
INTRODUCTION
The Generation of Genetically Modified Mice Using the CRISPR/Cas9 Genome Editing System
Adam Williams, Jorge Henao-Mejia, and Richard A. Flavell
PROTOCOL
Protocol for the Generation of Genetically Modified Mice Using the CRISPR/Cas9 Genome-Editing System
Jorge Henao-Mejia, Adam Williams, Anthony Rongvaux, Judith Stein,
Cynthia Hughes, and Richard A. Flavell
Chapter 11
INTRODUCTION
Genome Editing in Human Pluripotent Stem Cells
Cory Smith, Zhaohui Ye, and Linzhao Cheng
PROTOCOL
Genome Editing in Human Pluripotent Stem Cells
Cory Smith, Zhaohui Ye, and Linzhao Cheng
Chapter 12
INTRODUCTION
An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells
Dan Du and Lei S. Qi
PROTOCOL
CRISPR Technology for Genome Activation and Repression in Mammalian Cells
Dan Du and Lei S. Qi
APPENDIX
General Safety and Hazardous Material Information
Index
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