Flow Cytometry with Plant Cells Analysis of

Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.

Jaroslav Dolezel is the Head of the Czech Institute of Experimental Botany in Olomouc, and Associate Professor at the Palacky University in Olomouc. He has pioneered the use of flow cytometry in plant science for the analysis of nuclear genome size and regularly holds international training courses on the subject.

Johann Greilhuber is Associate Professor at the Institute of Botany at the University of Vienna. He has studied plant genomes for almost 30 years and is an expert on intraspecific variation of genome size. He has constantly been developing novel techniques to study plant genomes, among them many methods based on flow cytometry.

Jan Suda heads the laboratory of flow and image cytometry at the Czech Academy of Sciences' Institute of Botany. He is a taxonomist specializing in plant biosystematics, including genome size determination and has co-authored a book on taxonomy analysis.

Preface
List of Contributors
Cytometry and Cytometers: Development and Growth
Overview
Origins
From Absorption to Fluorescence, from Imaging to Flow
The Growth of Multiparameter Flow Cytometry
Bench-tops and Behemoths: Convergent Evolution
Image Cytometry: New Beginnings?
References
Principles of Flow Cytometry
Overview
Introduction
A Brief History of Flow Cytometry
Components of a Flow Cytometer
Flow Cytometric Informatics
Spectral Compensation
Cell Sorting
Calibration Issues
Conclusions
References
Flow Cytometry with Plants: an Overview
Overview
Introduction
Fluorescence is a Fundamental Parameter
Pushing Plants through the Flow Cytometer
Application of Flow Cytometry in Plants
A Flow Cytometer in Every Laboratory?
Conclusions and Future Trends
References
Nuclear DNA Content Measurement
Overview
Introduction
Nuclear DNA Content: Words, Concepts and Symbols
Units for Presenting DNA Amounts and their Conversion Factors
Sample Preparation for Flow Cytometric DNA Measurement
Standardization
Fluorescence Inhibitors and Coatings of Debris
Quality Control and Data Presentation
Future Directions
References
Flow Cytometry and Ploidy: Applications in Plant Systematics, Ecology and Evolutionary Biology
Overview
Introduction
Practical Considerations
Applications in Plant Systematics
Applications in Plant Ecology and Evolutionary Biology
Future Directions
References
Reproduction Mode Screening
Overview
Introduction
Analyses of the Mode of Reproduction
A Recent Innovative Method: the Flow Cytometric Seed Screen
Flow Cytometry with Mature Seeds for other Purposes
Conclusions
References
Genome Size and its Uses: the Impact of Flow Cytometry
Overview
Introduction
Why is Genome Size Important?
What is Known about Genome Size in Plants?
The Extent of Genome Size Variation across Plant Taxa
Understanding the Consequences of Genome Size Variation: Ecological and Evolutionary Implications
Methods of Estimating Genome Size in Plants and the Impact of Flow Cytometry
Recent Developments and the Future of Flow Cytometry in Genome Size Research
References
DNA Base Composition of Plant Genomes
Overview
Introduction
Analysis of Base Composition by Flow Cytometry
Conclusions
References
Detection and Viability Assessment of Plant Pathogenic Microorganisms using Flow Cytometry
Overview
Introduction
Viability Assessment
Immunodetection
Conclusions and Future Prospects
References
Protoplast Analysis using Flow Cytometry and Sorting
Overview
Introduction
Results of Protoplast Analyses using Flow Cytometry and Sorting
Walled P
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Flow Cytometry with Plant Cells Analysis of Genes, Chromosomes and Genomes

Summary

Author Biography

Table of Contents

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