Proteomic Biology Using LC/MS Large Scale

This is one of the first books to focus on the dynamic aspect of proteomes. The book introduces proteomics to the newcomer, reviews the theoretical aspects of proteomics and its state-of-the art technologies, along with a number of biological applications using "classical" proteomic technology. The book also presents a new concept, the Dynamome, or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events.

Nobuhiro Takahashi, PhD, is a Professor of Applied Biological Science in the Department of Agriculture at Tokyo University of Agriculture and Technology.

Toshiaki Isobe, PhD, is a Professor of Biochemistry in the Department of Chemistry at Tokyo Metropolitan University.

Introduction	p. vii
Overview of Proteomics	p. 1
What Is Proteomics?	p. 1
Establishment of Proteomics Using Mass Spectrometry	p. 3
Protein Separations by Two-Dimensional Electrophoresis	p. 3
Development of the Technologies for Protein Identification	p. 5
Protein Identification Based on Gel Separation and Mass Spectrometry	p. 6
Strategies for Characterizing Proteomes and Understanding Proteome Function	p. 15
Modification-Specific Proteomics	p. 17
Activity-Based Protein Profiling/Enzyme Substrate Proteomics	p. 31
Subcellular (Organellar) Proteomics	p. 41
Machinery or Complex Interaction Proteomics	p. 45
Dynamic Proteomics	p. 46
References	p. 49
Proteomic Tools for Analysis of Cellular Dynamics	p. 63
LC-Based Proteomics Technologies	p. 63
LC-MS system for Peptide Separation and Identification	p. 71
Application of LC-MS Methods to Functional Proteomics	p. 101
Development of Quantitative Proteomics	p. 131
Isotope Labeling for Quantitative Analysis Using MS	p. 132
Quantification Strategy for LC-MS Analysis of Isotope-Labeled Peptide Mixture and Software for Computer Analysis	p. 140
Label-Free Quantification Software	p. 145
Absolute Quantification	p. 147
References	p. 153
Dynamics of Functional Cellular Machinery: From Statics to Dynamics in Proteomic Biology	p. 167
Dynamic Analysis of Cellular Function	p. 167
Strategy for Dynamic Analysis of Cellular Machinery (Multiprotein Complexes)	p. 168
Methods for the Isolation of a Cellular Machine/Multiprotein Complex	p. 174
Cellular Machinery (Multiprotein Complex)	p. 196
Dynamics of Ribosome Biogenesis	p. 198
Snapshot Analysis of Preribosomal Particles in Yeast	p. 201
Snapshot Analysis of Preribosomal Particles in Mammals	p. 207
Quantitative (Dynamic) Analysis Using Isotope-Labeled Reagents	p. 224
Outline of Human/Mammalian Ribosome Biogenesis	p. 231
Dynamic Analysis of Subcellular Structures	p. 232
Proteome Dynamics of the Nucleolus	p. 233
References	p. 236
Index	p. 249
Table of Contents provided by Ingram. All Rights Reserved.

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Proteomic Biology Using LC/MS Large Scale Analysis of Cellular Dynamics and Function

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