2D Page

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

Preface	p. v
Contributors	p. xi
Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions	p. 1
Albumin and Immunoglobulin Depletion of Human Plasma	p. 15
Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum	p. 27
Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies	p. 41
Proteomics of Cerebrospinal Fluid: Methods for Sample Processing	p. 53
Sample Preparation of Bronchoalveolar Lavage Fluid	p. 67
Preparation of Nasal Secretions for Proteome Analysis	p. 77
Preparation of Urine Samples for Proteomic Analysis	p. 89
Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis	p. 101
Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells	p. 113
Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue: Proteomic Analysis of Formalin-Fixed Tissue	p. 131
Metalloproteomics in the Molecular Study of Cell Physiology and Disease	p. 139
Protein Extraction from Green Plant Tissue	p. 149
The Terminator: A Device for High-Throughput Extraction of Plant Material	p. 153
Isolation of Mitochondria from Plant Cell Culture	p. 163
Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants	p. 171
Isolation of Plant Cell Wall Proteins	p. 187
Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses	p. 203
Cell Wall Fractionation for Yeast and Fungal Proteomics	p. 217
Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration	p. 241
Sample Preparation Procedure for Cellular Fungi	p. 265
Isolation and Enrichment of Secreted Proteins from Filamentous Fungi	p. 275
Isolation and Solubilization of Cellular Membrane Proteins from Bacteria	p. 287
Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins	p. 295
Cell Fractionation of Parasitic Protozoa	p. 313
Index	p. 333
Table of Contents provided by Ingram. All Rights Reserved.

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