Alcamo’s Laboratory Fundamentals of Microbiology

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ISBN: 9780763726652 | 0763726656
Cover: Paperback
Copyright: 5/15/2004

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Updated with additional material for the new seventh edition, this popular lab manual offers thirty multi-part lab exercises designed to provide students with basic training in the handling of microorganisms, while exploring microbial properties and uses. This lab manual can also be used independently of the main text. A new instructor's manual, downloadable from the Web, now accompanies the lab manual and provides principles of lab safety; research topic ideas, information on customizing laboratory programs with the manual; helpful suggestions for setting up and running each exercise; and lists of laboratory media, cultures, and special materials used in each exercise. Contact your publishers representative for access information.Download the Instructors Lab Manual (105 KBytes)

Preface	p. vii
An Important Message About Safety	p. ix
Microscopy and Bacterial Staining Techniques	p. 1
The Compound Microscope	p. 3
Parts of the Microscope and Their Use	p. 3
Observations of Prepared Slides	p. 5
Preparation of a Bacterial Smear and the Simple Stain Technique	p. 11
Preparation of a Bacterial Smear	p. 11
The Simple Stain Technique	p. 14
The Negative Stain Technique	p. 19
The Gram Stain Technique	p. 25
Bacterial Structures	p. 31
Spore Stain Technique	p. 31
Capsule Stain Technique	p. 33
Bacterial Motility	p. 35
Culture Techniques and Unknown Identification	p. 41
Pure Culture Techniques	p. 43
Streak Plate Technique	p. 43
Pour Plate Technique	p. 46
Bacterial Culture Characteristics	p. 53
Biochemical Characteristics of Bacteria	p. 59
Carbohydrate Fermentation	p. 59
Starch Digestion	p. 60
Catalase Production	p. 62
DNA Digestion	p. 63
Hydrogen Sulfide Production	p. 64
Indole Production	p. 65
Urea Digestion	p. 66
Lipid Digestion	p. 67
Casein Digestion	p. 68
Identification of a Bacterial Unknown	p. 75
Viruses and Eukaryotic Microorganisms	p. 83
Viruses	p. 85
Constructing a Virus	p. 85
The Effect of Bacteriophages on Bacteria	p. 86
Plaque Formation	p. 88
Identification of Bacteriophages from Sewage	p. 89
Estimating the Number of Bacteriophages	p. 90
Fungi, Protozoa, and Multicellular Parasites	p. 97
Fungi (Molds and Yeasts)	p. 97
Protozoa and Multicellular Parasites	p. 102
Medical Microbiology	p. 109
The Genus Mycobacterium	p. 111
Colonial and Cellular Morphology	p. 111
Acid-Fast Stain Technique	p. 112
Cold Acid-Fast Stain Technique	p. 114
The Genus Streptococcus	p. 117
Streptococci from the Upper Respiratory Tract	p. 117
Streptococci from the Oral Cavity	p. 119
The Genus Neisseria	p. 125
The Genus Staphylococcus	p. 129
Isolation of Staphylococci	p. 129
Differentiation between Staphylococcal Species	p. 131
The Enteric Bacteria	p. 137
Isolation of Enteric Bacteria	p. 137
Differentiation of Enteric Bacteria on TSI Agar	p. 138
The IMViC Series	p. 140
Rapid Identification of Enteric Bacteria	p. 141
The Genus Bacillus	p. 149
The Genus Clostridium	p. 153
The Genus Lactobacillus	p. 159
Isolation of Lactobacilli	p. 159
Caries Susceptibility Test	p. 160
The Spread and Control of Microorganisms	p. 165
A Simulated Epidemic	p. 167
Microbial Transmission via Fomites	p. 167
A Microbial Hunt	p. 169
Transmission of Microorganisms through Toilet Paper	p. 171
The Effect of Physical Agents on Bacteria	p. 175
Heat Tolerance	p. 175
Ultraviolet Light	p. 177
Drying	p. 179
Other Physical Agents	p. 180
The Effect of Chemical Agents on Bacteria	p. 185
Antiseptics and Disinfectants	p. 185
Metals	p. 187
Other Chemical Substances	p. 188
Effect of Lysozyme on Bacteria	p. 189
Disinfection of Drinking Water	p. 190
Evaluation of Disinfectants and Antiseptics	p. 199
Effectiveness of Disinfectants on Inanimate Objects	p. 199
Effectiveness of Disinfectants and Hand Washing on the Skin Surface	p. 200
Mouthwashes and Oral Bacteria	p. 201
The Phenol Coefficient	p. 202
The Effect of Antibiotics on Bacteria	p. 209
Public Health and Environmental Microbiology	p. 219
Microbiology of Foods	p. 221
Food Preservation with Salt and Garlic	p. 221
Standard Plate Count of Food Products	p. 222
Fermentation of Sauerkraut	p. 225
Fermentation of Wine and Beer	p. 226
Microbiology of Milk and Dairy Products	p. 235
Standard Plate Count of Milk	p. 235
Coliform Plate Count of Milk	p. 237
Methylene Blue Reduction Test	p. 238
Preparation of Cheese	p. 239
Preparation of Yogurt	p. 240
The Natural Bacterial Content of Milk	p. 241
Microbiology of Water	p. 249
Presumptive Test by the MPN Method	p. 249
Confirmed Test	p. 250
Completed Test	p. 252
Membrane Filter Technique	p. 253
Preparation of a Biofilm	p. 254
Microbiology of Soil	p. 261
Isolation of Rhizobium from Legume Roots	p. 261
Ammonification by Soil Microorganisms	p. 262
Isolation of Streptomyces from Soil	p. 263
Antibiotic Production by Streptomyces	p. 263
Plate Count of Soil Bacteria	p. 265
Microbial Ecology in the Soil-The Winogradsky Column	p. 265
Recycling of Carbon in Food	p. 267
Recycling of Carbon in Cloth	p. 269
Recycling of Carbon in Leaves	p. 270
Bacterial Genetics and Serology	p. 279
Bacterial Genetics: Mutations and the Ames Test	p. 281
Gradient Plate Technique	p. 282
Mutagenic Effect of Ultraviolet Light	p. 283
DNA Repair of UV Light Damage	p. 285
The Ames Test	p. 286
Serology: Agglutination Techniques and Blood Cell Identification	p. 293
Slide Agglutination	p. 293
Tube Agglutination	p. 294
Hemagglutination: Determination of Blood Type	p. 296
Blood Smear	p. 298
Preparation of Stains and Diagnostic Reagents	p. 303
Using Enterotube II	p. 305
Index	p. 309
Table of Contents provided by Rittenhouse. All Rights Reserved.

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Alcamo’s Laboratory Fundamentals of Microbiology

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Table of Contents

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Alcamo’s Laboratory Fundamentals of Microbiology

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Author Biography

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