Applications of Flow Cytometry in Stem Cell

In most labs, training and resources are not available to teach methods of flow cytometry. Except for the original publications there are no books which talk about these sophisticated methods for stem cell analysis. There is need for a book which will review these procedures, discuss the science behind them, and show real examples to illustrate the usefulness of the methods. Besides using this book in work shops, it will be a valuable addition to any library or institution dealing in stem cell and tissue regeneration research.

Awtar Krishan, Ph.D is a Professor of Pathology at the University of Miami School of Medicine. From 1965 to 1977, he was at the Sidney Farber Cancer Institute, Harvard MedicalSchool as Head of the Tissue Ultrastructure and Experimental Pathology Labs. Some of his recent work has focused on development and applications of a new flow cytometer which can measure cell volume and marker expression of stem cells in human apheresis samples. Dr. Krishan has more than 160 publications in peer reviewed journals.

H. Krishnamurthy is working as Scientific Officer 'E' and in charge of Flow Cytometry and Confocal Microscopy Facilities at the National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. His research interest is hormonal regulation of spermatogenesis and trafficking and signaling of gonadotropin receptors.

Satish Totey is Chief Scientific Officer, Stempeutics Research Pvt. Ltd and Director at the Manipal Institute of Regenerative Medicine inBangalore. Dr. Totey was amongst the small number of researchers who pioneered the isolation and characterization of pluripotent stem cells from human embryos. The cell lines he established have been listed in the NIH stem cell registry. He has also developed up-scaling of mesenchymal stem cells and is currently conducting phase-I and II clinical trials for myocardial infarction, leg ischemia, cerebral stroke and Multiple Sclerosis. He has published 90 research papers and holds 12US patents.

Contributors	p. vii
Preface	p. xi
Basics of Flow Cytometry	p. 1
Practical Considerations for Flow Cytometric Sorting of Stem Cells	p. 13
Stem Cell Analysis and Sorting Using Side Population Analysis	p. 25
Flow Cytometry in the Study of Proliferation and Apoptosis	p. 45
Flow Cytometric Analysis of Drug Transport and Efflux in Stem Cells	p. 61
Stem Cell Biology and Application	p. 75
Identification and Isolation of Very Small Embryonic-like Stem Cells from Murine and Human Specimens	p. 91
Electronic Volume of Hematopoietic Stem Cells	p. 103
Hematopoietic Stem Cells: Issues in Enumeration	p. 115
Embryonic Stem Cells: Development and Characterization	p. 135
Human Embryonic Stem Cells: Long-Term Culture and Cardiovascular Differentiation	p. 161
Mesenchymal Stromal Cells and Their Clinical Applications	p. 175
Circulating Adult Stem Cells of Hematopoietic Origin for Vascular and Neural Regeneration	p. 189
Flow Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells	p. 211
Limbal Stem Cells and Corneal Regeneration	p. 223
Flow Cytometric Sorting of Spermatogonial Stem Cells	p. 241
Breast Cancer Stem Cells	p. 251
Tumor Stem Cell Marker Expression in Cells from Body Cavity Fluids	p. 271
Index	p. 279
Table of Contents provided by Ingram. All Rights Reserved.

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Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

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