Avidin-Biotin Interactions

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9781588295835 | 1588295834
Cover: Hardcover
Copyright: 2/8/2008

Rent

(Recommended)

$108.14

Term

Due

Price

Semester

Aug 2

$108.14

Quarter

Jul 19

$102.73

Short Term

Jun 21

$97.32

*This item is part of an exclusive publisher rental program and requires an additional convenience fee. This fee will be reflected in the shopping cart.
Buy Used

Usually Ships in 2-4 Business Days

$110.24
Buy New

Usually Ships in 7-10 Business Days

$147.75

This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.

Please wait while the item is added to your bag...

Preface	p. v
Contributors	p. xi
Preparation of Avidin Conjugates	p. 1
Coupling of Antibodies With Biotin	p. 13
Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin	p. 25
Use of the Avidin (Imino) Biotin System as a General Approach to Affinity Precipitation	p. 35
Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis	p. 51
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein	p. 63
Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives	p. 73
Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins	p. 89
High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli	p. 101
Endogenous Biotin in Rat Brain: Implications for False-Positive Results With Avidin-Biotin and Streptavidin-Biotin Techniques	p. 111
Pseudo-Immunolabelling With the Avidin-Biotin-Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina	p. 129
Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones	p. 139
Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay	p. 149
Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin	p. 157
Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure	p. 171
A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin	p. 187
Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications	p. 209
Index	p. 221
Table of Contents provided by Ingram. All Rights Reserved.

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Avidin-Biotin Interactions

Summary

Table of Contents

Supplemental Materials