Benson's Microbiological Applications Lab Manual

Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Microscopic MeasurementsPart 2 Survey of Microorganisms5 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria6 Ubiquity of Bacteria7 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms8 Aseptic Technique9 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms10 Smear Preparation11 Simple Staining12 Negative Staining13 Capsular Staining14 Gram Staining15 Spore Staining: Two Methods16 Acid-Fast Staining: Kinyoun Method17 Motility DeterminationPart 5 Culture Methods18 Culture Media Preparation19 Enumeration of Bacteria: The Standard Plate Count20 Slide Culture: FungiPart 6 Bacterial Viruses21 Determination of a Bacteriophage Titer22 Isolation of Phage from Flies23 Phage TypingPart 7 Environmental Influences and Control of Microbial Growth24 Effects of Oxygen on Growth25 Temperature: Effects on Growth26 pH and Microbial Growth27 Water Activity and Osmotic Pressure28 Ultraviolet Light: Lethal Effects29 The Effects of Lysozyme on Bacterial Cells30 Evaluation of Alcohol: Its Effectiveness as an Antiseptic31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method32 Evaluation of Antiseptics: The Filter Paper Disk Method33 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria34 Morphological Study of An Unknown Bacterium35 Cultural Characteristics36 Physiological Characteristics: Oxidation and Fermentation Stet Reactions37 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions38 Physiological Characteristics: Multiple Test Media39 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems40 Enterobacteriaceae Identification: The API 20E System41 Enterobacteriaceae Identification: The Entero-Pluri Test42 Staphylococcus Identification: The API Staph SystemPart 10 Applied Microbiology43 Bacterial Food Counts44 Bacteriological Examination of Water: Most Probable Number Determination45 Bacteriological Examination of Water: The Membrane Filter Method46 Reductase Test47 Temperature: Lethal Effects48 Microbial Spoilage of Canned Food49 Microbiology of Alcohol FermentationPart 11 Bacterial Genetics and Biotechnology50 Polymerase Chain51 Bacterial TransformationPart 12 Immunology and Serology52 The Staphylococci: Isolation and Identification53 The Streptococci and Enterococci: Isolation and Identification54 Gram-Negative Intestinal Pathogens55 A Synthetic EpidemicPart 13 Immunology and Serology56 Slide Agglutination Test: Serological Typing57 Slide Agglutination Test for S. aureus58 Slide Agglutination Test for Streptococcus59 Enzyme-Linked Immunosorbent Assay (ELISA)60 Blood GroupingAppendicesAppendix A TablesAppendix B Indicators, Stains, ReagentsAppendix C MediaAppendix D Identification Charts