Benson's Microbiological Applications Laboratory Manual (2025 Evergreen Release)

• Part 1 Microscopy
• 1. Brightfield Microscopy
• 2. Darkfield Microscopy
• 3. Phase-Contrast Microscopy
• 4. Microscopic Measurements
• Part 2 Survey of Microorganisms
• 5. Microbiology of Pond Water - Protists, Algae, and Cyanobacteria
• 6. Ubiquity of Bacteria
• 7. The Fungi: Molds and Yeasts
• Part 3 Manipulation of Microorganisms
• 8. Aseptic Technique
• 9. Pure Culture Techniques
• Part 4 Staining and Observations of Microorganisms
• 10. Smear Preparation
• 11. Simple Staining (Observing Bacterial Cell Morphology)
• 12. Negative Staining
• 13. Capsular Staining
• 14. Gram Staining
• 15. Endosphere Staining
• 16. Acid-Fast Staining: Kinyoun Method
• 17. Motility Determination
• Part 5 Culture Methods
• 18. Culture Media Preparation
• 19. Enumeration of Bacteria: The Standard Plate Count
• 20. Slide Culture: Fungi
• Part 6 Bacterial Viruses
• 21. Determination of a Bacteriophage Titer
• 22. Isolation of Phage from Flies
• 23. Phage Typing
• Part 7 Environmental Influences and Control of Microbial Growth
• 24. Effects of Oxygen on Growth
• 25. Temperature: Effects on Growth
• 26. pH and Microbial Growth
• 27. Water Activity and Osmotic Pressure
• 28. Ultraviolet Light: Lethal Effects
• 29. The Effects of Lysozyme on Bacterial Cells
• 30. Evaluation of Alcohol: Its Effectiveness as an Antiseptic
• 31. Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
• 32. Evaluation of Antiseptics: The Filter Paper Disk Method
• 33. Effectiveness of Hand Scrubbing
• Part 8 Identification of Unknown Bacteria
• 34. Morphological Study of an Unknown Bacterium
• 35. Cultural Characteristics
• 36. Physiological Characteristics: Oxidation and Fermentation Tests
• 37. Physiological Characteristics: Hydrolytic and Degradative Reactions
• 38. Physiological Characteristics: Multiple Test Media
• 39. Use of Bergey’s Manual
• Part 9 Miniaturized Multitest Systems
• 40. Enterobacteriaceae Identification: The API 20E® System
• 41. Enterobacteriaceae Identification: The Entero-Pluri- Test System
• 42. Staphylococcus Identification: The API® Staph Test System
• Part 10 Applied Microbiology
• 43. Bacterial Counts of Foods
• 44. Bacteriological Examination of Water: Most Probable Number Determination
• 45. Bacteriological Examination of Water: The Membrane Filter Method
• 46. Reductase Test
• 47. Temperature: Lethal Effects
• 48. Microbial Spoilage of Canned Food
• 49. Microbiology of Alcohol Fermentation
• Part 11 Bacterial Genetics and Biotechnology
• 50. Polymerase Chain Reaction for Amplifying DNA
• 51. Bacterial Transformation
• 52. CRISPR-Cas9 System
• Part 12 Immunology and Serology
• 53. The Staphylococci: Isolation and Identification
• 54. The Streptococci and Enterococci: Isolation and Identification
• 55. Gram-Negative Intestinal Pathogens
• 56. A Synthetic Epidemic
• Part 13 Immunology and Serology
• 57. Slide Agglutination Test: Serological Typing
• 58. Slide Agglutination Test for S. aureus
• 59. Slide Agglutination Test for Streptococcus
• 60. Enzyme-Linked Immunosorbent Assay (ELISA)
• 61. Blood Grouping

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