- ISBN: 9780125181242 | 0125181248
- Cover: Paperback
- Copyright: 8/1/1992
Preface | p. ix |
Note to the reader | p. xiii |
Glossary | p. xv |
Chemiosmotic Energy Transduction | p. 3 |
Introduction | p. 3 |
The chemiosmotic theory: fundamentals | p. 3 |
The basic morphology of energy-transducing membranes | p. 7 |
Overview | p. 14 |
Ion Transport Across Energy-Conserving Membranes | p. 17 |
Introduction | p. 17 |
The classification of ion transport | p. 17 |
Bilayer-mediated transport | p. 21 |
Protein-catalysed transport | p. 25 |
Swelling and the co-ordinate movement of ions across membranes | p. 26 |
Quantitative Bioenergetics: the Measurement of Driving Forces | p. 31 |
Introduction | p. 31 |
Gibbs energy and displacement from equilibrium | p. 33 |
Oxidation-reduction (redox) potentials | p. 40 |
Ion electrochemical potential differences | p. 46 |
Photons | p. 47 |
Bioenergetic interconversions and thermodynamic constraints on their stoichiometries | p. 48 |
The equilibrium distributions of ions, weak acids and weak bases | p. 50 |
Membrane potentials, diffusion potentials, Donnan potentials and surface potentials | p. 52 |
The Chemiosmotic Proton Circuit | p. 57 |
Introduction | p. 57 |
The measurement of protonmotive force | p. 59 |
The stoichiometry of proton extrusion by the respiratory chain | p. 66 |
The stoichiometry of proton uptake by the ATP synthase | p. 68 |
Proton current and respiratory control | p. 69 |
Proton conductance | p. 73 |
Mitochondrial respiration rate and metabolic control analysis | p. 77 |
Overall parameters of energy transduction | p. 81 |
Reversed electron transfer and the proton circuit driven by ATP hydrolysis | p. 83 |
ATP synthesis driven by an artificial protonmotive force | p. 85 |
Kinetic competence of [Delta]p in the proton circuit | p. 86 |
Light-dependent ATP synthesis by bovine heart ATP synthase | p. 87 |
Respiratory Chains | p. 89 |
Introduction | p. 89 |
Components of the mitochondrial respiratory chain | p. 89 |
The sequence of redox carriers in the respiratory chain | p. 95 |
The mechanism of electron transfer | p. 99 |
Proton translocation by the respiratory chain: 'loops', 'conformation pumps' or both? | p. 105 |
Complex I (NADH-UQ oxidoreductase) | p. 107 |
Delivering electrons to ubiquinone without proton translocation | p. 111 |
Ubiquinone and complex III (bc[subscript 1] or UQ-cyt c oxidoreductase) | p. 114 |
Cytochrome c and complex IV (cytochrome c oxidase; ferrocytochrome c: O[subscript 2] oxidoreductase) | p. 119 |
Overall proton and charge movements catalysed by the respiratory chain: correlation with the P/O ratio | p. 126 |
Superoxide production by complexes I and III | p. 127 |
Oxidative stress | p. 129 |
The nicotinamide nucleotide transhydrogenase | p. 130 |
Electron transport in mitochondria of non-mammalian cells | p. 131 |
Bacterial respiratory chains | p. 134 |
Photosynthetic Generators of Protonmotive Force | p. 157 |
Introduction | p. 157 |
The light reaction of photosynthesis in Rhodobacter sphaeroides and related organisms | p. 159 |
The generation by illumination or respiration of [Delta]p in photosynthetic bacteria | p. 168 |
The electron-transfer and light-capture pathway in green plants and algae | p. 171 |
Bacteriorhodopsin and halorhodopsin | p. 186 |
The ATP Synthase | p. 195 |
Introduction | p. 195 |
F[subscript 1] and F[subscript o] | p. 195 |
The subunits of the F[subscript 1].F[subscript o]-ATPase | p. 198 |
The structure of F[subscript 1].F[subscript o] | p. 199 |
Enzymology of ATP synthase | p. 204 |
Relating the structure to function for ATP synthase | p. 210 |
Non-thermodynamic regulation of the ATP synthase | p. 216 |
Proton translocation by other ATPases and pyrophosphatases | p. 216 |
Metabolite and Ion Transport | p. 219 |
Introduction | p. 219 |
Mitochondrial cation transporters | p. 220 |
Mitochondrial metabolite transporters | p. 225 |
The transfer of electrons from cytoplasmic NADH to the respiratory chain | p. 229 |
The phosphate and adenine nucleotide transporters | p. 230 |
The uncoupling protein family | p. 232 |
Bacterial transport | p. 234 |
Transport (movement) of bacterial cells | p. 246 |
Transport of macromolecules across bacterial membranes | p. 247 |
Mitochondria in the Cell | p. 249 |
Introduction | p. 249 |
Monitoring [Delta psi subscript m] and ATP synthesis in intact cells | p. 251 |
Mitochondria and cellular Ca[superscript 2+] homeostasis | p. 255 |
Mitochondria and programmed cell death | p. 258 |
Mitochondria and necrotic cell death | p. 261 |
The mitochondrial genome | p. 263 |
Import and assembly of mitochondrial proteins | p. 264 |
Mitochondrial genetic diseases | p. 266 |
Mitochondrial involvement in neurodegenerative diseases | p. 268 |
References | p. 271 |
Protein structures | p. 283 |
Index | p. 287 |
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