Biological Applications of Microfluidics

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ISBN: 9780470074831 | 0470074833
Cover: Hardcover
Copyright: 2/15/2008

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Microfluidics has numerous potential applications in biotechnology, pharmaceuticals, the life sciences, defense, public health, and agriculture. This book details recent advances in the biological applications of microfluidics, including cell sorting, DNA sequencing on-a-chip, microchip capillary electrophoresis, and synthesis on a microfluidic format. It covers microfabricated LOC technologies, advanced microfluidic tools, microfluidic culture platforms for stem cell and neuroscience research, and more. This is an all-in-one, hands-on resource for analytical chemists and researchers and an excellent text for students.

Preface
Contributors
Microfluidics
Microfluidics
Cell Analysis On Microfluidic Devices
Using Microfluidics to Understand and Control the Cellular Microenvironment
Introduction: Engineering the Microenvironment
The Chemical Microenvironment
The Mechanical Microenvironment
Conclusion
Microfabricated Devices for Cell Sorting
Introduction
Microfabricated Formats for Cell Sorting
Outlook for the Future
Advanced Microfluidic Tools for Single-Cell Manipulation and Analysis
Introduction
Fluidic Control
Temperature Control
Cell Manipulation
Detection
Integration
Conclusions
Engineering Cellular Microenvironments with Microfluidics
Introduction
Microfluidic Cultures can Simulate in vivo Microenvironments
Other Useful Capabilities of Microfluidic Cell Culture Devices
Microfluidic Devices Useful for Cell Applications Other than Culture
Future Prospects for Biological Studies in Microfluidic Bioreactors
Microfluidic Culture Platforms for Stem Cell and Neuroscience Research
Introduction
Applications for Stem Cell Research
Applications for Neuroscience Research
Summary and Future Directions
Enzymatic And Nonenzymatic Reactions On Microchips
Microfluidics for Studying Enzyme Inhibition
Enzyme Assays and Inhibition
Microfluidic Assays for Enzymes and Enzyme Inhibition
Enzyme Inhibition Studies in Microfluidic Devices: Specific Studies
Chemical Synthesis within Continuous Flow Microreactors
Introduction
Advantages of Performing Chemical Synthesis in Microreactors
Chemical Synthesis in Microreactors
Large-Scale Manufacture Using Microreactors
Conclusions
Microfluidic Reactors for Sequential and Parallel Reactions
Introduction
Sequential Reactions in Microfluidic Devices
Parallel Reactions in Microfluidic Devices
Conclusions
Gene Isolation, Gene Transformation, and Enzyme Reaction on a Chip
Introduction
DNA/RNA Isolation on a Microfluidic Chip
Gene Ligation on a Microfluidic Chip
Gene Transformation on a Chip
Enzymatic Reaction on a Chip
Summary and Perspective
Separations On Microchips
Chemical Monitoring in Complex Biological Environments Using Separation-Based Sensors in Chips
Separation-Based Sensors
Fast Separations with Separation-Based Sensors
Micro Total Analysis Systems with Electrophoretic Separations for Monitoring of Biological Systems
Miniaturization and Integration of Separation-Based Sensor Components
Analytical Strategies Toward the Analysis of Phenolic Compounds
Electrophoresis
Introduction
Experimental Section
Results and Discussion
Applications
Conclusions
Chemical Separations in 3D Microfluidics
Introduction
Fabrication
Results and Discussion on 3D Valves
Microfluidic Three-Dimensional Separation Columns
Results on Liquid Chromatography
Conclusions
Enabling Fundamental Research in Proteomics
Introduction
Membrane Protein Extraction
Conclusion
Biomedical Applications Of Microfluidics
Microengineering Neural Development
Introduction
Microengineering Guidance of Axons to their Targets
Synaptogenesis on a Microfluidic Chip
Conclusions
Applications of Centrifugal Microfluidics in Biology
Introduction
Why Use Centrifugal Force for Fluid Manipulation?
How Centrifugal Microfluidic Platforms Work
CD Applications
Conclusions
Microfluidic Techniques for Point-of-Care In Vitro Diagnostics
Introduction
Microfluidic Immunoassays
Microfluidic Vias and Derivative Applications
Conclusions
Microfluidic Fabrication Studies
Fabrication of Polymeric Microfluidic Devices
Introduction
Glass- and Silicon-Based Materials
Plastics and Polymeric Materials
Approaches to Microfabrication
Selected Microfabrication Techniques
Conclusions
Nano Fountain Pen: Toward Integrated, Portable, Lab-on-Chip Devices
Introduction
Nano Fountain Pen
Protein Printing
Enzyme Lithography
Polymer Microlenses
Conclusions
Surface Engineering of Microfluidic Devices Using Reactive Polymer Coatings
Introduction
Microfluidics Surface Modification Techniques
Conclusions
Microchips Containing In Situ Patterned Polymeric Media for Biochemical Analysis
Introduction and Scope
General Information about Patterned Materials
Photopatterned Materials for Protein Analysis
DNA Purification and Analysis
Patterned Materials for Cell Culture and Analysis
Other Biomolecules
Conclusions
Hybrid Microfluidic Applications
Coupling Electrochemistry to Microfluidics
Introduction
Electrochemical Methods of Analysis
Microfluidic Devices
Applications
Conclusions and Future Directions
Manipulating Mass-Limited Samples Using Hybrid Microfluidic/Nanofluidic Networks
Introduction
Nanofluidics
Hybrid Microfluidic/Nanofluidic Systems
Functionalized NCAMs
The Future
Magnetic Bead-based Methods to Study the Interaction of Teicoplanin with Peptides and Bacteria
Introduction
Experimental
Results and Discussion
Conclusions
Acknowledgments
Interfacing Microchannel Electrophoresis with Electrospray Ionization Mass Spectrometry
Introduction
Electrospray Ionization
Coatings
Spray Emitters
CE and ESI Electrode Connections
Integrated Applications
Conclusions
Index
Table of Contents provided by Publisher. All Rights Reserved.

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Biological Applications of Microfluidics

Summary

Author Biography

Table of Contents

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