Circadian Rhythms

Circadian Rhythms: Methods and Protocols is an in-depth examination of circadian biology, presented by leading researchers in the field. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. These robust, reproducible procedures take into account the myriad of variables that circadian researchers must be cognizant of while conducting their research. By distilling the arsenal of methodologies available, and presenting essential procedures in precise detail, Circadian Rhythms: Methods and Protocols supports a vast majority of circadian research efforts. Part I of this seven-part volume provides a concise introduction to essential components of circadian biology, including light, photoreceptors, circadian clocks, and statistical analysis. Part II presents protocols and techniques for monitoring and analyzing rhythmic patterns of several model organisms. Parts III, IV, and V emphasize the identification of clock genes and protein and RNA expression in circadian biology. Protocols for in vitro systems comprise Part VI, and Part VII provides an outline of methods and tips for microscopy analysis. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection of methods and protocols for the study of circadian rhythms.