Clinical Flow Cytometry

, by Bauer, Kenneth D.; Duque, Ricardo E.; Shankey, T. Vincent

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ISBN: 9780683004809 | 0683004808
Cover: Hardcover
Copyright: 1/1/1993

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Preface
Acknowledgments
Contributors
Principles of Clinical Flow Cytometry
Theoretical Aspects
DNA Content as a Genetic Marker of Cancer Cells	p. 3
The Cell Cycle: Application of Flow Cytometry in Studies of Cell Reproduction	p. 13
Theoretical Aspects of Flow Cytometry Data Analysis	p. 41
Flow Cytometry Reveals the Complexity and Diversity of the Human Immune System	p. 63
Technical Aspects
Clinical Flow Cytometry Instrumentation	p. 71
Technical Considerations for Dissociation of Fresh and Archival Tumors	p. 93
Fluorescence Reagents for Flow Cytometry	p. 111
Practical Considerations for DNA Content and Cell Cycle Analysis	p. 117
Cytochemistry I: Cell Surface Immunofluorescence	p. 143
Cytochemistry II: Immunofluorescence Measurement of Intracellular Antigens	p. 157
Quality Control for Clinical Flow Cytometry	p. 177
Clinical Application
Applications in Clinical Oncology
Lymphomas	p. 203
Acute Leukemias	p. 235
Breast Cancer	p. 247
Clinical Commentary	p. 260
Gynecological Cancer	p. 263
Clinical Commentary	p. 269
Urological Cancers	p. 271
Clinical Commentary	p. 301
Colorectal Neoplasia	p. 307
Clinical Commentary	p. 316
Upper Aerodigestive and Lower Respiratory Tract Tumors	p. 319
Clinical Commentary	p. 328
Pediatric Solid Tumors	p. 331
Soft-Tissue Sarcomas	p. 343
Clinical Commentary	p. 356
Relative Applicability of Image Analysis and Flow Cytometry in Clinical Medicine	p. 359
Applications in Clinical Hematology and Immunology
Flow Cytometric Analysis of Red Blood Cells	p. 373
Flow Cytometric Analysis of Platelets	p. 387
Flow Cytometric Analysis of Granulocytes	p. 405
Role of Flow Cytometric Evaluation of Congenital and Acquired Immunodeficiencies	p. 435
Role of Flow Cytometry in Clinical Transplantation	p. 449
Emerging Clinical and Research Applications
Flow Cytometric Monitoring of Cellular Resistance to Cancer Chemotherapy	p. 459
Cell-Associated Receptor Quantitation	p. 469
Detection of Minimal Residual Disease by Flow Cytometry	p. 479
Flow Cytometric Assays of Cell-Mediated Cytotoxicity	p. 497
Measurements of Cell Physiology: Ionized Calcium, pH, and Glutathione	p. 505
Microsphere-Based Fluorescence Immunoassays Using Flow Cytometry Instrumentation	p. 535
Flow Cytometry in Skin Disorders	p. 545
Molecular Biology and Flow Cytometry I: Analytical Considerations	p. 557
Molecular Biology and Flow Cytometry II: Clinical Potential	p. 573
Expert Systems for Cytometry Data Analysis	p. 585
Appendix: Workshop-Defined Cluster Groups for Monoclonal Antibodies to Human Leukocytes	p. 603
Index	p. 609
Table of Contents provided by Blackwell. All Rights Reserved.

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