Clinical Virology Manual

The definitive clinical virology resource for physicians and clinical laboratory virologists

The clinical virology field is rapidly evolving and, as a result, physicians and clinical laboratory virologists must have a reliable reference tool to aid in their ability to identify and diagnose viral infections to prevent future outbreaks.

In this completely revised edition of the Clinical Virology Manual, Editor in Chief, Michael Loeffelholz, along with Section Editors, Richard Hodinka, Benjamin Pinsky, and Stephen Young, have complied expert perspectives of a renowned team of clinical virology experts and divided these contributions into three sections to provide

• the latest information on the diagnosis of viral infections, including ebola, HIV and Human papillomavirus
• state of the art diagnostic technologies, including next-generation sequencing and nucleic acid amplification methods
• taxonomy of clinically important viruses such as polyomaviruses and zoonotic viruses

This comprehensive reference also includes three appendices with vital information on reference virology laboratories at the Centers for Disease Control and Prevention, state and local public health laboratories, and international reference laboratories and laboratory systems.

Additionally, a new section "Diagnostic Best Practices," which summarizes recommendations for diagnostic testing, and cites evidence-based guidelines, is included in each viral pathogens chapter.

Clinical Virology Manual, Fifth Edition serves as a reference source to healthcare professionals and laboratorians in providing clinical and technical information regarding viral diseases and the diagnosis of viral infections.

Contributors

Author Biographies

Preface to the Fifth Edition

Section I General Topics in Clinical Virology

1 The Taxonomy, Classification, and Characterization of Medically Important Viruses 3
Steven J. Drews

2 Quality Assurance and Quality Control in Clinical and Molecular Virology 27
Matthew J. Bankowski

3 Regulatory Compliance 35
Linoj Samuel

4 Laboratory Safety 41
K. Sue Kehl

5 Laboratory Design 51
Matthew J. Binnicker

Section II Laboratory Procedures For Detecting Viruses

6 Specimen Requirements Selection, Collection, Transport, and Processing 59
Reeti Khare and Thomas E. Grys

7 Primary Isolation of Viruses 79
Marie L. Landry and Diane Leland

8 Viral Antigen Detection 95
Diane S. Leland and Ryan F. Relich

9 Serologic (Antibody Detection) Methods 105
Dongxiang Xia, Debra A. Wadford, Christopher P. Preas, and David P. Schnurr

10 Nucleic Acid Extraction in Diagnostic Virology 117
Raymond H. Widen and Suzane Silbert

11 Nucleic Acid Amplification by Polymerase Chain Reaction 129
Ana María Cárdenas and Kevin Alby

12 Isothermal Nucleic Acid Amplification Methods 137
Harald H. Kessler and Evelyn Stelzl

13 Quantitative Molecular Methods 145
Natalie N. Whitfield and Donna M. Wolk

14 Signal Amplification Methods 167
Yun (Wayne) Wang

15 DNA Sequencing for Clinical and Public Health Virology: Some Assembly Required 173
Joanne Bartkus

16 Phenotypic and Genotypic Antiviral Susceptibility Testing 201
Martha T. Van Der Beek and Eric C. J. Claas

17 Point-of-Care Diagnostic Virology 229
James J. Dunn and Lakshmi Chandramohan

18 Future Technology 243
Erin Mcelvania Tekippe and Carey-Ann D. Burnham

Section III Viral Pathogens

19 Respiratory Viruses 257
Christine Robinson, Michael J. Loeffelholz, and Benjamin A. Pinsky

20 Enteroviruses and Parechoviruses 277
M. Steven Oberste and Mark A. Pallansch

21 Measles, Mumps, and Rubella Viruses 293
William J. Bellini, Joseph P. Icenogle, and Carole J. Hickman

22 Gastrointestinal Viruses 311
Michael D. Bowen

23 Hepatitis A and E Viruses 329
Gilberto Vaughan and Michael A. Purdy

24 Hepatitis B and D Viruses 341
Rebecca T. Horvat

25 Hepatitis C Virus 351

Melanie Mallory and David Hillyard

26 Herpes Simplex Viruses and Varicella Zoster Virus 363
Scott H. James and Mark N. Prichard

27 Cytomegalovirus 373
Preeti Pancholi and Stanley I. Martin

28 Epstein-Barr Virus 387
Derrick Chen and Belinda Yen-Lieberman

29 Human Herpesviruses 6, 7, and 8 399
Sheila C. Dollard and Timothy M. Karnauchow

30 Human Papillomaviruses 413
Susan Novak-Weekley and Robert Pretorius

31 Human Polyomaviruses 427
Rebecca J. Rockett, Michael D. Nissen, Theo P. Sloots, and Seweryn Bialasiewicz

32 Parvoviruses 443
Richard S. Buller

33 Poxviruses 457
Ashley V. Kondas and Victoria A. Olson

34 Rabies Virus 473
Robert J. Rudd and April A. Davis

35 Arboviruses 493
Laura D. Kramer, Elizabeth B. Kauffman, Norma P. Tavakoli

36 Animal-Borne Viruses 515
Gregory J. Berry, Michael J. Loeffelholz, and Gustavo Palacios

37 Human Immunodeficiency Viruses and Human T-lymphotropic Viruses 527
Jörg Schüpbach

38 Chlamydiae 545
Barbaravan Der Pol and Charlotte A. Gaydos

39 The Human Virome 561
Matthew C. Ross, Nadim J. Ajami, and Joseph F. Petrosino

40 Human Susceptibility and Response to Viral Diseases 567
Ville Peltola and Jorma Ilonen

Appendixes: Reference Virology Laboratories

Appendix 1 Reference Virology Laboratory Testing Performed at the Centers for Disease Control 581
Roberta B. Carey

Appendix 2 Public Health Laboratory Virology Services 585
Jane Getchell

Appendix 3 International Reference Laboratories Offering Virology Services 595
Ariel I. Suarez and Cristina Videla

Index 607

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.