Current Protocols Essential Laboratory

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Sean R. Gallagher, Ph.D. is the Chief Technology Officer and Director of Product Development for UVP, Inc. (Upland, CA), where he oversees applied technology and product development for specialty light source and life science products. He is on the faculty of the Keck Graduate Institute of Applied Life Sciences and Harvey Mudd College (Claremont, CA) and holds eight patents on technology he developed. He received his doctorate in Botany from the University of California at Riverside and completed a post-doctoral position at Stanford University.

Emily A. Wiley, Ph.D. is an Associate Professor of Biology in the Joint Science Department of the Claremont-McKenna, Scripps, and Pitzer Colleges, Claremont, CA. She received her doctorate in Molecular Genetics from the University of Washington. She is actively involved in graduate and undergraduate teaching courses and is committed to providing proper training to those students. She has an active research program studying chromatin assembly in Tetrahymena thermophila.

Foreword
Preface
Contributors List
Conversion FactorsFlow Chart: Application of Techniques
Volume/Weight Measurement
Volume Measurement
Weight Measurement
Concentration Measurement
Spectrophotometry
Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Reagent Preparation
Theoretical Discussion
Practical Discussion
Recipes In-House
Cell Culture Techniques
Aseptic Technique
Culture of Escherichia coli and Related Bacteria
Sample Preparation
Centrifugation and Cell Fractionation In-House
Purification and Concentration of Nucleic Acids
Chromatography
Overview of Chromatography
Column Chromatography
Electrophoresis
Overview of Electrophoresis
Agarose Gel Electrophoresis
SDS-PAGE
Staining of Gels
Blotting
Overview of Blotting
Nucleic Acid Blotting: Southern and Northern
Protein Blotting: Immunoblotting
Labeling DNA and Probe Preparation
Microscopy
Conventional Light Microscopy
Immunofluorescence Microscopy
Enzymatic Reactions
Working with Enzymes
Overview of PCR
Real-Time PCR
Bioinformatics
Using NCBI Blast
Table of Contents provided by Publisher. All Rights Reserved.

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Current Protocols Essential Laboratory Techniques

Summary

Author Biography

Table of Contents

Supplemental Materials