Dendritic Cell Protocols : Methods in

Univ. College Hospital, London, UK. Features state-of-the-art collection of laboratory techniques, with readily reproducible techniques detailed step-by-step. Also gives proven methods for isolating dendritic cells from a wide variety of tissues. DNLM: Dendritic Cells--physiology.

Foreword

v

Preface

vii

Contributors

xiii

Part I Isolation of Dendritic Cells from Animal Tissues

Isolation of Dendritic Cells from Mouse Lymph Nodes

3

(6)

Dmitry Gabrilovich

Isolation of Mouse Spleen Dendritic Cells

9

(14)

Andrew J. Stagg

Fiona Burke

Suzanne Hill

Stella C. Knight

Isolation of Mouse Thymic Dendritic Cells

23

(6)

Fabienne Anjuere

Carlos Ardavin

Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen

29

(14)

Gordon MacPherson

Michelle Wykes

Fang-Ping Huang

Chris Jenkins

Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells

43

(20)

Franz Koch

Eckhart Kampgen

Gerold Schuler

Nikolaus Romani

Murine Intestinal Dendritic Cell Isolation

63

(10)

Paul Pavli

Identification and Isolation of Rodent Lung Dendritic Cells

73

(12)

Philip A. Stumbles

Jennifer Thomas

Patrick G. Holt

Isolation and Propagation of Mouse Liver-Derived Dendritic Cells

85

(14)

Lina Lu

Maureen L. Drakes

Angus W. Thomson

Part II Isolation and Characterization of Human Dendritic Cells

Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells

99

(12)

Stephen P. Robinson

Purification of Dendritic Cells from Peripheral Blood

111

(10)

Steven Patterson

Aaron Rae

Heather Donaghy

Isolation of Human Tonsillar Dendritic Cells

121

(16)

Paul U. Cameron

Gianna Stent

Isolation of Dendritic Cells from Human Afferent Lymph

137

(8)

Christoph U. Brand

Nikhil Yawalkar

Robert E. Hunger

Lasse R. Braathen

Isolation of Human Skin Dendritic Cells by In Vitro Migration

145

(10)

Cornelia D. Richters

Matthias J. Hoekstra

John S. du Pont

Robert W. Kreis

Eduard W. A. Kamperdijk

Human Intestinal Dendritic Cell Isolation

155

(8)

Paul Pavli

Isolation of Human Lung Dendritic Cells

163

(12)

Michel M. van den Heuvel

Carin E. G. Havenith

Robert H. J. Beelen

Identification and Isolation of Synovial Dendritic Cells

175

(16)

Allison R. Pettit

Lois Cavanagh

Ranjeny Thomas

Part III Derivation of Animal Dendritic Cells from Progenitor Cells

Generation of Murine Bone-Marrow-Derived Dendritic Cells

191

(8)

Asher Maroof

Generation of Dendritic Cells from Rat Bone Marrow

199

(8)

Timothy Powell

James R. Major

Gordon MacPherson

Derivation of Murine Dendritic Cells from Thymic Precursors

207

(12)

Karen Lucas

Dolores Saunders

Li Wu

Part IV Generation of Murine Dendritic Cell Lines

Generation of Mouse Dendritic Cell Lines

219

(12)

Stefania Citterio

Maria Rescigno

Maria Foti

Francesca Granucci

Malgosia Matyszak

Giampiero Girolomoni

Paola Ricciardi-Castagnoli

Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin

231

(12)

Thilo Jakob

Mark C. Udey

Derivation of Dendritic Cell Lines from Mouse Skin

243

(14)

Akira Takashima

Paul R. Bergstresser

Part V Generation of Human Dendritic Cells from Progenitor Cells

Propagation of Human Dendritic Cells In Vitro

257

(18)

Christophe Caux

Bertrand Dubois

Generation of Dendritic Cells from Lymphoid Precursors

275

(8)

Anne Galy

Dendritic Cell Generation from Highly Purified CD14+ Monocytes

283

(14)

Winfried F. Pickl

Otto Majdic

Walter Knapp

Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy

297

(10)

Michel F. Gilliet

Fank O. Nestle

Part VI Aspects of Dendritic Cell Function

Chemotaxis of In Vitro Cultured Human Dendritic Cells

307

(6)

Walter Luini

Giovanna D'Amico

Sergio Bernasconi

Paola Allavena

Alberto Mantovani

Silvano Sozzani

Adhesion of Dendritic Cells to Endothelia

313

(12)

K. Alun Brown

Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells

325

(6)

Giancarlo Bianchi

Giovanna D'Amico

Silvano Sozzani

Alberto Mantovani

Paola Allavena

In Vivo Assays of Langerhans Cell Migration

331

(16)

Marie Cumberbatch

Rebecca J. Dearman

Ian Kimber

In Vitro Migration of Rat Dendritic Cells

347

(22)

Kenjiro Matsuno

Taichi Ezaki

Antigen Uptake by Dendritic Cells

369

(8)

Christophe Caux

Bertrand Dubois

Uptake of Antigen---Antibody Complexes by Human Dendritic Cells

377

(10)

Neil A. Fanger

Paul M. Guyre

Robert F. Graziano

Immunoelectron Microscopy of Antigen Processing in Dendritic Cells

387

(26)

Monique J. Kleijmeer

George Posthuma

Hans J. Geuze

Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells

413

(10)

Philippe Pierre

Detection of Intracellular Cytokines in Dendritic Cells

423

(6)

Peter Kelleher

Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells

429

(12)

Wenru Song

Ronald G. Crystal

Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells

Xiaoli Huang

441

(14)

Zheng Fan

Lian Zheng

Charles R. Rinaldo, Jr.

Index

455

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Dendritic Cell Protocols : Methods in Molecular Medicine

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Table of Contents

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Dendritic Cell Protocols : Methods in Molecular Medicine

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Table of Contents

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