Diagnostic Bacteriology Protocols

This fully updated edition includes the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically- and immunologically-based formats that can provide significant potential for multiparameter testing and automation.

Preface

v

Contributors

ix

1 A Review of Current and Future Molecular Diagnostic Tests for Use in the Microbiology Laboratory

Geert Jannes and Daniel De Vos

1

(22)

2 Overview of DNA Purification for Nucleic Acid-Based Diagnostics From Environmental and Clinical Samples.,

Knut Rudi and Kjetill S. Jakobsen

23

(14)

3 Microarray-Based Detection of Bacteria by On-Chip PCR

Georg Mitterer and Wolfgang M. Schmidt

37

(16)

4 An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods

Miriam M. Ngundi and Chris R. Taitt

53

(16)

5 Detection of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae in Blood and Cerebrospinal Fluid Using Fluorescence-Based PCR

Stuart C. Clarke

69

(10)

6 Use of Hybridization Probes in a Real-Time PCR Assay on the LightCycler® for the Detection of Methicillin-Resistant Staphylococcus aureus

Andrea J. Grisold and Harald H. Kessler

79

(12)

7 Detection of Verotoxin Genes VT 1 and VT 2 in Escherichia colt O157:H7 in Minced Beef Using Immunocapture and Real-Time PCR

Justine Fitzmaurice

91

(6)

8 Application of Two-Step Quantitative Reverse-Transcription PCR to Bacterial Diagnostics

Barry Glynn

97

(14)

9 Quencher Extension for Single Nucleotide Polymorphism Quantification in Bacterial Typing and Microbial Community Analyses

Knut Rudi, Monika Zimonja, and Beate Skånseng

111

(8)

10 Amplified Fragment-Length Polymorphism and Protein Profiling for Identification of Campylobacter lari Subgroups

Birgitta Duim and Jaap Wagenaar

119

(12)

11 Use of Peptide Nucleic Acid Probes for Rapid Detection and Enumeration of Viable Bacteria in Recreational Waters and Beach Sand

Nwadiuto Esiobu

131

(10)

12 HOOF-Prints: Brucella Strain Typing by PCR Amplification of Multilocus Tandem-Repeat Polymorphisms

Betsy J. Bricker and Darla R. Ewalt

141

(34)

13 Detection of Legionella in Various Sample Types Using Whole-Cell Fluorescent In Situ Hybridization

Priscilla Declerck and Frans Ollevier

175

(10)

14 Identification of Diagnostic Proteins in Mycobacterium avium subspecies paratuberculosis by a Whole Genome Analysis Approach

John P. Bannantine and Michael L. Paustian

185

(12)

15 Diagnosis of Q Fever Using Indirect Microimmunofluorescence

Guenievre Imbert and Bernard La Scola

197

(6)

16 Macrophage Cell Cultures for Rapid Isolation of Intracellular Bacteria: The Mycobacterium bovis Model

Massimo Amadori, Marco Ritelli, Silvia Tagliabue, and Maria L. Pacciarini

203

(16)

Index

219

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Diagnostic Bacteriology Protocols

Summary

Table of Contents

Supplemental Materials