Differential Display Methods and Protocols

Drs. Peng Liang and Arthur B. Pardee assemble for the first time a comprehensive review of the state of the art of their powerful new methodology and practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. They also provide numerous examples in which differentially expressed genes were successfully identified in diverse biological systems ranging from plants to songbirds to humans.

Methodologies. Differential Display-A General Protocol Peng Liang and Arthur B. Pardee. Fingerprinting by Arbitrarily Primed PCR M. McClelland Rhonda Honeycutt Francoise Mathieu-Daude Thomas Vogt and John Welsh. Differential Display Using Random Hexamer-Primed cDNA Motif Primers and Agarose Gel Electrophoresis Patrick J. Donohue Debbie K. W. Hsu and Jeffrey A. Winkles. Fluorescent Differential Display Takashi Ito and Yoshiyuki Sakaki. Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization Jackson S. Wan and Mark G. Erlander. Direct Sequencing of Differential Display PCR Products Xinkang Wang and Giora Z. Feuerstein. A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels Katherine J. Martin Chi-Pong Kwan and Ruth Sager. Differential Screening of Differential Display cDNA Products by Reverse Northern Hong Zhang Rong Zhang and Peng Liang. Screening for Positive Clones Generated by Differential Display Regina Vgeli-Lange Niels B?rckert Thomas Boller and Andres Wiemken. Cloning Family Specific Genes. Cloning of the 3' Noncoding Regions From Several Members of Heat Shock Protein Gene Families by Differential Display Chandrashekhar P. Joshi and Henry T. Nguyen. RC4D-Restriction Fragment Length Polymorphism-Coupled Domain- Directed Differential Display G?nter Theiben and Achim Fischer. Cloning Inducible Genes. Identification of Immediate-Early Gene Targets of the Raf-1 Serine/Threonine Protein Kinase Using an Estradiol-Dependent Fusion Protein DRaf-1:ER Sean A. McCarthy Natasha Aziz and Martin McMahon. Isolation of Cytokine-Inducible Genes from Hemapoietic Cells by Differential Display Yuan Zhu Tom Hahn and Alan D. D'Andrea. Hormone-Inducible Genes in Prostate Cells Lidia Averboukh Peng Liang Stephen A. Douglas and Arthur B. Pardee. Cloning Differentially Expressed Genes During Normal Development. Application of Differential Display in Studying Developmental Processes M. Jorge Guimaraes Terrill McClanahan and J. Fernando Bazan. Identification of Novel Genes in Adipose Differentiation by Differential Display Erding Hu and Bruce M. Spiegelman. Isolation of Song-Regulated Genes in the Brain of Songbirds Claudio V. Mello Erich D. Jarvis Natalia Denisenko and Miriam Rivas. Cloning Differentially Expressed Genes in Diseases. Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display Carla B. Green and Joseph C. Besharse. Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues Mary E. Russell Anne R"is"nen-Sokolowskind and Ulrike Utans. Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model SystemWilliam D. Pennie Yi Sun Hyungtae Kim and Nancy H. Colburn. Identification of Genes Associated With Resistance to Photodynamic Therapy Mediated Oxidative Stress Charles J. Gomer Marian C. Luna and Angela Ferrario. RAP-PCR Using RNA from Tissue Microdissection Roy A. Jensen David L. Page and Jeffrey T. Holt. The Application of Differential Display to the Brain: Adaptations for the Study of Heterogeneous Tissue Joseph M. Babity Richard A. Newton Mario E. Guido and Harold A. Robertson. Analysis of Gene Expression in Hypothalamus in Obese and Normal Mice Using Differential Display Eleftheria Maratos-Flier Daqing Qu and Steen Gammeltoft. Index.