DNA Cloning A Practical Approach Volume 2:

Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Screening expression libraries with antibody and protein probes
Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
Purification of over-produced proteins from E.coli cells
Production of monoclonal antibodies against proteins expressed in E.coli
Expression of cloned genes in yeast
Intraction trap cloning with yeast
The baculovirus expression system
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DNA Cloning A Practical Approach Volume 2: Expression Systems

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Table of Contents

Supplemental Materials