Emerging Technology Platforms for Stem Cells

, by Lakshmipathy, Uma; Chesnut, Jonathan D.; Thyagarajan, Bhaskar

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ISBN: 9780470146934 | 0470146931
Cover: Hardcover
Copyright: 4/6/2009

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This book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with their use for drug delivery.

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Foreword	p. ix
Preface	p. xi
Contributors	p. xiii
	p. 1
Derivation Methods for Human Embryonic Stem Cells: Past, Present, and Future	p. 3
Isolation of Human Embryonic Stem Cells from Various Stages of the Human Embryo	p. 19
Derivation of Stem Cells from Epiblasts	p. 29
Derivation of Embryonic Stem Cells from Parthenogenetic Eggs	p. 39
Reprogramming Developmental Potential	p. 51
	p. 87
Adult Stem Cells and Their Role in Endogenous Tissue Repair	p. 89
Greater Differentiation Potential of Adult Stem Cells	p. 111
Cancer Stem Cells	p. 129
Large-Scale Production of Adult Stem Cells for Clinical Use	p. 153
	p. 169
Genetic and Epigenetic Features of Stem Cells	p. 171
Directed Differentiation of Embryonic Stem cells	p. 203
Identification of Signaling Pathways Involved During Differentiation	p. 235
	p. 249
Media and Extra Cellular Matrix Requirements for Large-Scale ESC Growth	p. 251
Automated Method for Embryonic Stem Cell Culture	p. 275
Quantitative 2D Imaging of Human Embryonic Stem Cells	p. 283
Nanobiotechnology for Stem Cell Culture and Maintenance	p. 291
Engineering Microenvironments to Control Stem Cell Functions	p. 311
	p. 329
Improved Lentiviral Gene Delivery Tools for Stem Cells	p. 329
Sleeping Beauty-Mediated Transposition in Stem Cells	p. 341
PhiC31 Integrase for Modification of Stem Cells Wonil	p. 363
Cell Engineering Using Integrase and Recombinase Systems	p. 379
	p. 395
Human Embryonic Stem Cell-Derived Cardiomyocytes for Cell Therapy and Drug Discovery	p. 397
Human Embryonic Stem Cells in Drug Discovery	p. 417
Characterization and Culturing of Adipose-Derived Precursor Cells	p. 439
Bringing Mesenchymal Stem Cells into the Clinic	p. 463
Index	p. 483
Table of Contents provided by Ingram. All Rights Reserved.

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Emerging Technology Platforms for Stem Cells

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Emerging Technology Platforms for Stem Cells

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Author Biography

Table of Contents

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