Flow Cytometry Protocols

In addition to providing chapters on the theoretical and practical aspects of flow cytometry this book contains protocols used specifically for immediate use in the diagnostic and experimental measurements enabled by modern flow cytometric instrumentation. Research protocols cover cell cycle analysis and regulation detection of rare cells solid tumor DNA techniques ploidy analysis and the application of flow cytometric methods for measurements of leukemias necrosis apoptosis heat shock proteins liver and bladder cancer disease monitoring and intracellular measurements. Hematologic and immunologic applications are also described by researchers with a particular expertise in their use. A special section is included for biotechnologists using flow cytometry in the study of protein secretion the monitoring of fermentation processes monitoring bioreactors cell-cell electroporation quantitation electrofusion and electrochemotherapy.

Basics of Flow Cytometry G.Radcliff and M. J. Jaroszeski. Detection of Terminal Transferase in Leukemia E. Paietta. The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells B. E. Crucian and R. H. Widen. Detection of Intracellular/Intranuclear Antigens R. H. Widen. Detection of Intracellular/Intranuclear Antigens in Tumor Cells R. H. Widen and J. L. Becker. Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry G. Juan and Z. Darzynkiewicz. Estimation of Cell Viability by Flow Cytometry J. L. Weaver. Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential M. K. Tanner and S. R. Wellhausen. Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH A. Imrich and L. Kobzik. Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry B. Stringer and L. Kobzik. Estimation of Drug Resistance by Flow Cytometry A. Aszalos and J. L. Weaver. Flow Cytometric Monitoring of Cellular Pharmacokinetics M. S. Dordal. Quantification of Xenoprotein Electroinsertion in Mammalian Cells K. El Ouagari and J. Teissi'. Flow Cytometry Quantification of Electropermeabilization M.-P. Rols and J. Teissi'. Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products M. J. Jaroszeski R. Gilbert and R. Heller. Cell Cycle Analysis of Asynchronous Populations M. G. Ormerod. Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies K. Steck and A. K. El-Naggar. Solid Tumor DNA Ploidy Analysis K. Steck and A. K. El-Naggar. FISHing for Cytokines: Methodology Combining Flow Cytometry and In Situ Hybridization K. J. Pennline. Analysis of Apoptosis by Flow Cytometry W. Gorczyca M. R. Melamed and Z. Darzynkiewicz. Chromosome Sorting and Analysis by FACS S. P. Monard. Simultaneous Five-Six Color Multiparameter Analysis K. J. Pennline. Index.